Anti-Kv1.6/RCK2 抗体

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Kv1.6/RCK2 antibody (AB21089)

ICC/IF image of ab21089 stained human HEK (Human embryonic kidney) 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab21089, 5μg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

• WB

Project

Western blot - Anti-Kv1.6/RCK2 antibody (AB21089)

ab21089 recognises a band corresponding to the predicted MW of Kv1.6/RCK2 in mouse brain lysate (lane 1). Antibody binding to this band may be blocked using the immunising peptide, ab23431 (lane 2). The antibody also recognises additional proteins. These may be degradation/cleavage products of Kv1.6/RCK2, or they may represent non-specific binding by the antibody.

All lanes:

Western blot - Anti-Kv1.6/RCK2 antibody (ab21089) at 1 µg/mL

Lane 1:

Mouse Brain Lysate at 20 µg

Lane 2:

Mouse Brain Lysate Kv1.6/RCK2 at 20 µg with Human Kv1.6/RCK2 peptide (ab23431)

Secondary

Lane 1:

Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution

Lane 2:

Alexa Fluor Goat polyclonal to Rabbit IgG

Predicted band size: 59 kDa

Observed band size: 35 kDa,39 kDa,45 kDa,58 kDa

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