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リコンビナント

Anti-Kv1.1 potassium channel 抗体 [K36/15] (ab300664)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)
  • Immunohistochemistry (Frozen sections) - Anti-Kcna1 antibody [K36/15] (AB300664)
  • Immunohistochemistry (Frozen sections) - Anti-Kcna1 antibody [K36/15] (AB300664)
  • Anti-Kcna1 antibody [K36/15] (AB300664)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [K36/15] to Kv1.1 potassium channel
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Kv1.1 potassium channel antibody [K36/15]
    Kv1.1 potassium channel 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [K36/15] to Kv1.1 potassium channel

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, IHC-Frmore details
    適用なし: WB

  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Mouse and rat hippocampus, FFPE tissue sections. IHC-Fr: Rat hippocampus, fresh frozen tissues.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300664の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/10000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/500.

Not Applicable.
特記事項
IHC-P
1/10000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/500.

Not Applicable.
追加情報
Is unsuitable for WB.

ターゲット情報

  • 機能

    Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

  • 関連疾患

    Defects in KCNA1 are the cause of episodic ataxia type 1 (EA1) [MIM:160120]; also known as paroxysmal or episodic ataxia with myokymia (EAM) or paroxysmal ataxia with neuromyotonia. EA1 is an autosomal dominant disorder characterized by brief episodes of ataxia and dysarthria. Neurological examination during and between the attacks demonstrates spontaneous, repetitive discharges in the distal musculature (myokymia) that arise from peripheral nerve. Nystagmus is absent.
    Defects in KCNA1 are the cause of myokymia isolated type 1 (MK1) [MIM:160120]. Myokymia is a condition characterized by spontaneous involuntary contraction of muscle fiber groups that can be observed as vermiform movement of the overlying skin. Electromyography typically shows continuous motor unit activity with spontaneous oligo- and multiplet-discharges of high intraburst frequency (myokymic discharges). Isolated spontaneous muscle twitches occur in many persons and have no grave significance.

  • 配列類似性

    Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.1/KCNA1 sub-subfamily.

  • ドメイン

    The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

  • 翻訳後修飾

    Palmitoylated on Cys-243; which may be required for membrane targeting.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • AEMK antibody
      • EA1 antibody
      • Episodic ataxia with myokymia antibody
      • HBK1 antibody
      • HUK1 antibody
      • Kca1 1 antibody
      • Kcna1 antibody
      • KCNA1_HUMAN antibody
      • Kcpvd antibody
      • KV1.1 antibody
      • MBK1 antibody
      • mceph antibody
      • MGC124402 antibody
      • MGC126782 antibody
      • MGC138385 antibody
      • MK1 antibody
      • MK1, mouse, homolog of KV1.1 antibody
      • Potassium channel protein 1 antibody
      • Potassium voltage gated channel shaker related subfamily member 1 antibody
      • Potassium voltage gated channel subfamily A member 1 antibody
      • Potassium voltage gated channel, shaker related subfamily, member 1 (episodic ataxia with myokymia) antibody
      • Potassium voltage-gated channel subfamily A member 1 antibody
      • RBK1 antibody
      • RCK1 antibody
      • Shak antibody
      • Shaker related subfamily member 1 antibody
      • Voltage gated potassium channel subunit Kv1.1 antibody
      • Voltage-gated K(+) channel HuKI antibody
      • Voltage-gated potassium channel HBK1 antibody
      • Voltage-gated potassium channel subunit Kv1.1 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)

      Immunohistochemical analysis of paraffin-embedded mouse hippocampus labeling Kcna1 with ab300664 at 1/10000 dilution (0.099 µg/ml) followed by mouse IgG1 antibody (ab125913) for 8 mins and a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on mouse hippocampus. The section was incubated with ab300664 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody without primary.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)

      Immunohistochemical analysis of paraffin-embedded mouse liver labeling Kcna1 with ab300664 at 1/10000 dilution (0.099 µg/ml) followed by mouse IgG1 antibody (ab125913) for 8 mins and a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Negative control: no staining on mouse liver. The section was incubated with ab300664 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody without primary.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)

      Immunohistochemical analysis of paraffin-embedded rat hippocampus labeling Kcna1 with ab300664 at 1/10000 dilution (0.099 µg/ml) followed by mouse IgG1 antibody (ab125913) for 8 mins and a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on rat hippocampus. The section was incubated with ab300664 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody without primary.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)

      Immunohistochemical analysis of paraffin-embedded rat liver labeling Kcna1 with ab300664 at 1/10000 dilution (0.099 µg/ml) followed by mouse IgG1 antibody (ab125913) for 8 mins and a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Negative control: no staining on rat liver. The section was incubated with ab300664 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody without primary.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Frozen sections) - Anti-Kcna1 antibody [K36/15] (AB300664)
      Immunohistochemistry (Frozen sections) - Anti-Kcna1 antibody [K36/15] (AB300664)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized fresh frozen sectioned rat hippocampus tissue labeling Aquaporin 1 with ab300664 at 1/500 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution (green). Positive staining on rat hippocampus is observed. The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Secondary antibody without adding primary.

      No antigen retrieval was carried out.

    • Immunohistochemistry (Frozen sections) - Anti-Kcna1 antibody [K36/15] (AB300664)
      Immunohistochemistry (Frozen sections) - Anti-Kcna1 antibody [K36/15] (AB300664)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized fresh frozen sectioned rat liver tissue labeling Aquaporin 1 with ab300664 at 1/500 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution (green). Negative control (PMID: 24309898) - no staining on rat liver is observed. The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Secondary antibody without adding primary.

      No antigen retrieval was carried out.

    • Anti-Kcna1 antibody [K36/15] (AB300664)
      Anti-Kcna1 antibody [K36/15] (AB300664)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300664 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300664 は論文での使用が確認できていません。

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