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Anti-Kv1.1 potassium channel 抗体 (ab65790)

Reviews (1)Q&A (2)References (2)
Western blot - Anti-Kv1.1 potassium channel antibody (ab65790)

    Key features and details

    • Rabbit polyclonal to Kv1.1 potassium channel
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-Kv1.1 potassium channel antibody [EPR12718] (ab177481)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-Kv1.1 potassium channel antibody
      Kv1.1 potassium channel 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to Kv1.1 potassium channel

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat

    • 免疫原

      Synthetic peptide derived from the C terminal domain of human Kv1.1 potassium channel.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab65790の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/1000 - 1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
    特記事項
    WB
    1/1000 - 1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).

    ターゲット情報

    • 機能

      Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

    • 関連疾患

      Defects in KCNA1 are the cause of episodic ataxia type 1 (EA1) [MIM:160120]; also known as paroxysmal or episodic ataxia with myokymia (EAM) or paroxysmal ataxia with neuromyotonia. EA1 is an autosomal dominant disorder characterized by brief episodes of ataxia and dysarthria. Neurological examination during and between the attacks demonstrates spontaneous, repetitive discharges in the distal musculature (myokymia) that arise from peripheral nerve. Nystagmus is absent.
      Defects in KCNA1 are the cause of myokymia isolated type 1 (MK1) [MIM:160120]. Myokymia is a condition characterized by spontaneous involuntary contraction of muscle fiber groups that can be observed as vermiform movement of the overlying skin. Electromyography typically shows continuous motor unit activity with spontaneous oligo- and multiplet-discharges of high intraburst frequency (myokymic discharges). Isolated spontaneous muscle twitches occur in many persons and have no grave significance.

    • 配列類似性

      Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.1/KCNA1 sub-subfamily.

    • ドメイン

      The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
      The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

    • 翻訳後修飾

      Palmitoylated on Cys-243; which may be required for membrane targeting.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • AEMK antibody
      • EA1 antibody
      • Episodic ataxia with myokymia antibody
      • HBK1 antibody
      • HUK1 antibody
      • Kca1 1 antibody
      • Kcna1 antibody
      • KCNA1_HUMAN antibody
      • Kcpvd antibody
      • KV1.1 antibody
      • MBK1 antibody
      • mceph antibody
      • MGC124402 antibody
      • MGC126782 antibody
      • MGC138385 antibody
      • MK1 antibody
      • MK1, mouse, homolog of KV1.1 antibody
      • Potassium channel protein 1 antibody
      • Potassium voltage gated channel shaker related subfamily member 1 antibody
      • Potassium voltage gated channel subfamily A member 1 antibody
      • Potassium voltage gated channel, shaker related subfamily, member 1 (episodic ataxia with myokymia) antibody
      • Potassium voltage-gated channel subfamily A member 1 antibody
      • RBK1 antibody
      • RCK1 antibody
      • Shak antibody
      • Shaker related subfamily member 1 antibody
      • Voltage gated potassium channel subunit Kv1.1 antibody
      • Voltage-gated K(+) channel HuKI antibody
      • Voltage-gated potassium channel HBK1 antibody
      • Voltage-gated potassium channel subunit Kv1.1 antibody
      see all

    画像

    • Western blot - Anti-Kv1.1 potassium channel antibody (ab65790)
      Western blot - Anti-Kv1.1 potassium channel antibody (ab65790)
      All lanes : Anti-Kv1.1 potassium channel antibody (ab65790) at 1/500 dilution

      Lane 1 : Human brain tissue lysate - total protein (ab29466)
      Lane 2 : Human testis tissue lysate - total protein (ab30257)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 57 kDa
      Observed band size: 57 kDa
      Additional bands at: 73 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 3 minutes

    参考文献 (2)

    ab65790 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab65790 は 2 報の論文で使用されています。

    • Viader A  et al. Schwann cell mitochondrial metabolism supports long-term axonal survival and peripheral nerve function. J Neurosci 31:10128-40 (2011). IHC-FoFr ; Mouse . PubMed: 21752989
    • Heeroma JH  et al. Episodic ataxia type 1 mutations differentially affect neuronal excitability and transmitter release. Dis Model Mech 2:612-9 (2009). ICC/IF ; Rat . PubMed: 19779067

    レビューと Q&A

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    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Kv1.1 potassium channel antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Zebra finch Cell (Brain sections)
    Permeabilization
    Yes - 0.3% Triton-X
    Specification
    Brain sections
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Mar 27 2019

    Question

    Dear NuLife, I need clarifications regarding some Abcam antibodies. I wish to do immunohistochemistry on formalin fixed paraffin embedded tissues and am not clear which would be the best suited for the purpose. 1. Anti-beta catenin - I want to use an antibody which will predominantly give nuclear immunopositivity on FFPEs. I want the antibody to be against CTNNB1 and use it for medulloblastomas. The following catalogue nos. are listed of which you please suggest the best one for the purpose : AB22656 AB6301 AB49459 In case there is any other better antibody for Beta Catenin, please suggest. 2. GRM8 - There are 2 catlague nos. AB53094 and AB13194 Please indicate, which is better for FFPEs. Also, please indicate if you have any monoclonal antibody for this which works on FFPE. 3. KCNA1 - Please indicate if you have any monoclonal antibody for this which works on FFPE. 4. GLI1, SFRP1 and NPR3 - Please indicate if you have any monoclonal antibody for these three which works on FFPE. Thanks and Best wishes.

    Read More
    Answer

    Thank you for contacting us. Having checked our catalog for the appropriate products, I would like to send you the following recommendations. Where possible I looked for both monoclonal and polyclonal antibodies, so if not mentioned we unfortunately do not have monoclonals. I am happy to let you know that all tested species and applications are mentioned on the datasheet, and we can guarantee the antibody to work as indicated. In case the application IHC-P (or the species human) is not mentioned, the customer is eligible for our testing discount program:The discount would be to the value of 1 free primary antibody, if the customer would like to test the antibody in IHC-P/human (www.abcam.com/collaborationdiscount). 1. Anti-beta catenin: ab22656: Click here (or use the following: https://www.abcam.com/beta-Catenin-antibody-12F7-ab22656.html). ab16051: Click here (or use the following: https://www.abcam.com/beta-Catenin-antibody-ab16051.html). 2. GRM8 ab53094: Click here (or use the following: https://www.abcam.com/MGLUR8-antibody-ab53094.html). ab55067 (testing discount IHC-P): Click here (or use the following: https://www.abcam.com/MGLUR8-antibody-ab55067.html). 3. KCNA1 ab99010 (testing discount human): Click here (or use the following: https://www.abcam.com/Kv11-potassium-channel-antibody-S36-15-ab99010.html). ab32433: Click here (or use the following: https://www.abcam.com/Kv11-potassium-channel-antibody-ab32433.html). 4. GLI1, SFRP1 and NPR3: ab92611 (no monoclonals available): Click here (or use the following: https://www.abcam.com/Gli1-antibody-ab92611.html). ab4193 (no monoclonals available): Click here (or use the following: https://www.abcam.com/SFRP1-antibody-ab4193.html). ab123957: Click here (or use the following: https://www.abcam.com/Natriuretic-Peptide-Receptor-C-antibody-OTI11H6-ab123957.html). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Thank you for your kind help. I will try using 5% milk, if i will have more problems i will contact you again and in the case that it's necessary changing the product.  About this possibility, i need to know if the change must be with the same product or if it's possible a change with an another antibody,  because i saw you offer various anti Kv1.1. In the case that we decide to ask a change do i have to contact you? Thanks again in advance

    Read More
    Answer

    Thanks for your continued troubleshooting efforts. I look forward to hearing how things go. If you end up needing a replacement antibody, you can select any primary antibody from our catalog as a replacement. You are not limited to only another vial of ab65790.

    Read More

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