Anti-Ku70 + Ku80 抗体 - C-terminal (ab166822)
Key features and details
- Rabbit polyclonal to Ku70 + Ku80 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Ku70 + Ku80 antibody - C-terminal
Ku70 + Ku80 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Ku70 + Ku80 - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to a region within the C terminal of Human Ku70 + Ku80 (P12956, P13010).
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ポジティブ･コントロール
- LOVO cell extracts; Human kidney tissue; A549 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
without Mg2+, Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab166822の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 70, 82 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/500.
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 70, 82 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/500. |
ターゲット情報
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関連性
The Ku autoantigen is a heterodimer of 70kDa (p70) and 80kDa (p80) proteins. The p70 / p80 dimer is important for function of a 460kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. The Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 2547 Human
- Entrez Gene: 7520 Human
- Omim: 152690 Human
- Omim: 194364 Human
- SwissProt: P12956 Human
- SwissProt: P13010 Human
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別名
- CTC75 antibody
- CTC85 antibody
- CTCBF antibody
see all
画像
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Western blot - Anti-Ku70 + Ku80 antibody - C-terminal (ab166822)All lanes : Anti-Ku70 + Ku80 antibody - C-terminal (ab166822) at 1/500 dilution
Lane 1 : LoVo cell lysate
Lane 2 : LoVo cell lysate with synthesized peptide
Predicted band size: 70, 82 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 + Ku80 antibody - C-terminal (ab166822)Immunohistochemical analysis of formalin fixed, paraffin embedded Human kidney tissue labeling Ku70 + Ku80 with ab166822 at 5 µg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-Ku70 + Ku80 antibody - C-terminal (ab166822)Immunofluorescence analysis of A549 cells labeling Ku70 + Ku80 with ab166822 at 1/100. The panel on the right is treated with the synthesized peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab166822 は論文での使用が確認できていません。