Anti-Ku70 + Ku80 抗体 (ab53126)
Key features and details
- Rabbit polyclonal to Ku70 + Ku80
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Ku70 + Ku80 antibody
Ku70 + Ku80 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Ku70 + Ku80 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
A synthetic peptide derived from human Ku70 + Ku80.
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ポジティブ･コントロール
- LoVo cell extracts and human breast carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab53126 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53126の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | ||
| IHC-P |
| 特記事項 |
|---|
追加情報
ELISA: 1/20000.
IHC-P: 1/50 - 1/100.
WB: 1/300 - 1/1000. Detects bands of approximately 70 and 83 kDa (predicted molecular weight: 70 and 83 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
WB: 1/300 - 1/1000. Detects bands of approximately 70 and 83 kDa (predicted molecular weight: 70 and 83 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性
The Ku autoantigen is a heterodimer of 70kDa (p70) and 80kDa (p80) proteins. The p70 / p80 dimer is important for function of a 460kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. The Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 2547 Human
- Entrez Gene: 7520 Human
- Entrez Gene: 14375 Mouse
- Entrez Gene: 22596 Mouse
- Entrez Gene: 25019 Rat
- Entrez Gene: 363247 Rat
- Omim: 152690 Human
- Omim: 194364 Human
see all -
別名
- CTC75 antibody
- CTC85 antibody
- CTCBF antibody
see all
画像
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Western blot - Anti-Ku70 + Ku80 antibody (ab53126)All lanes : Anti-Ku70 + Ku80 antibody (ab53126) at 1/300 dilution
Lane 1 : LoVo cell extract
Lane 2 : LoVo cell extract with immunising peptide
Predicted band size: 70 , 83 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 + Ku80 antibody (ab53126)ab53126 at 1/50 dilution staining Ku70 + Ku80 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue in the absence and presence of the immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab53126 は 4 報の論文で使用されています。
- Luo Y et al. ß-catenin nuclear translocation induced by HIF-1a overexpression leads to the radioresistance of prostate cancer. Int J Oncol 52:1827-1840 (2018). PubMed: 29658569
- King HO et al. RAD51 Is a Selective DNA Repair Target to Radiosensitize Glioma Stem Cells. Stem Cell Reports 8:125-139 (2017). WB . PubMed: 28076755
- Marampon F et al. Hypoxia sustains glioblastoma radioresistance through ERKs/DNA-PKcs/HIF-1a functional interplay. Int J Oncol 44:2121-31 (2014). PubMed: 24676782
- Sun L et al. Characterization of the interaction of Aha1 with components of the Hsp90 chaperone machine and client proteins. Biochim Biophys Acta 1823:1092-101 (2012). PubMed: 22504172