Anti-KRTHA3B 抗体 (ab154339)
Key features and details
- Rabbit polyclonal to KRTHA3B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-KRTHA3B antibody -
製品の詳細
Rabbit polyclonal to KRTHA3B -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 140-404 of Human KRTHA3B (Uniprot ID: Q14525)
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ポジティブ･コントロール
- HeLa cells and whole cell lysate, paraffin-embedded Cal27 xenograft
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154339の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/5000 - 1/20000. Predicted molecular weight: 46 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/5000 - 1/20000. Predicted molecular weight: 46 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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配列類似性
Belongs to the intermediate filament family. - Information by UniProt
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参照データベース
- Entrez Gene: 3884 Human
- Omim: 602762 Human
- SwissProt: Q14525 Human
- Unigene: 32950 Human
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別名
- HA 3II antibody
- HA3II antibody
- Hair keratin antibody
see all
画像
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Western blot - Anti-KRTHA3B antibody (ab154339)Anti-KRTHA3B antibody (ab154339) at 1/10000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 46 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-KRTHA3B antibody (ab154339)Immunofluorescence analysis of methanol-fixed HeLa cells labeling KRTHA3B with ab154339 at 1/500 dilution (green) and counterstained with Hoechst 3342 in bottom panel (blue). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRTHA3B antibody (ab154339)Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, labeling KRTHA3B with ab154339 at 1/500 dilution
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab154339 は論文での使用が確認できていません。