Anti-KRT6B 抗体 (ab154313)
Key features and details
- Rabbit polyclonal to KRT6B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-KRT6B antibody
KRT6B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KRT6B -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment corresponding to a region within amino acids 134-564 of Human KRT6B.
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ポジティブ･コントロール
- HeLa whole cell lysate. HeLa cells. Mouse brain tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154313の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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組織特異性
Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath. -
関連疾患
Defects in KRT6B are a cause of pachyonychia congenita type 2 (PC2) [MIM:167210]; also known as pachyonychia congenita Jackson-Lawler type. PC2 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma and hyperhidrosis, follicular hyperkeratosis, multiple epidermal cysts, absent/sparse eyebrow and body hair, and by the presence of natal teeth. -
配列類似性
Belongs to the intermediate filament family. - Information by UniProt
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参照データベース
- Entrez Gene: 3854 Human
- Entrez Gene: 16688 Mouse
- Omim: 148042 Human
- SwissProt: P04259 Human
- SwissProt: Q9Z331 Mouse
- Unigene: 708950 Human
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別名
- CK-6B antibody
- CK6B antibody
- Cytokeratin-6B antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6B antibody (ab154313)Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling KRT6B using ab154313 at 1/500 dilution.
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Western blot - Anti-KRT6B antibody (ab154313)Anti-KRT6B antibody (ab154313) at 1/5000 dilution + HeLa cell lysate at 1 µg
Predicted band size: 60 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-KRT6B antibody (ab154313)Immunofluorescence analysis of methanol-fixed HeLa cells, labeling KRT6B using ab154313 at 1/500 dilution. Right panel costained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154313 は 1 報の論文で使用されています。
- Moses RL et al. Novel epoxy-tiglianes stimulate skin keratinocyte wound healing responses and re-epithelialization via protein kinase C activation. Biochem Pharmacol 178:114048 (2020). PubMed: 32446889