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リコンビナントRabMAb

Anti-KPNA4 抗体 [EPR26347-21] (ab302556)

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Western blot - Anti-KPNA4 antibody [EPR26347-21] (AB302556)
  • Western blot - Anti-KPNA4 antibody [EPR26347-21] (AB302556)
  • Western blot - Anti-KPNA4 antibody [EPR26347-21] (AB302556)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA4 antibody [EPR26347-21] (AB302556)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA4 antibody [EPR26347-21] (AB302556)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA4 antibody [EPR26347-21] (AB302556)
  • Immunoprecipitation - Anti-KPNA4 antibody [EPR26347-21] (AB302556)
  • Anti-KPNA4 antibody [EPR26347-21] (AB302556)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26347-21] to KPNA4
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-KPNA4 antibody [EPR26347-21]
    KPNA4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26347-21] to KPNA4

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, IPmore details
    適用なし: ICC/IF

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Whole cell lysates: 293T (human embryonic kidney epithelial cell), A549 (human lung carcinoma epithelial cell), NIH/3T3 (mouse embryonic fibroblast), L-929 (mouse connective tissue fibroblast), HeLa (human cervix adenocarcinoma epithelial cell), Jurkat (human T cell leukemia cell line from peripheral blood), Raji (human Burkitt's lymphoma b lymphocyte). Human, mouse and rat testis and mouse and rat ovary tissue lysates, human kidney. IHC-P: Human: lung, testis, and colon carcinoma tissues. IP: A549.

  • 特記事項

    ab302556 does not react in immunocytochemistry with human and mouse and in IHC-P with mouse. 

     

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR26347-21

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302556の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IHC-P
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
特記事項
WB
1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IHC-P
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
追加情報
Is unsuitable for ICC/IF.

ターゲット情報

  • 機能

    Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.

  • 組織特異性

    Highly expressed in testis, ovary, small intestine, heart, skeletal muscle, lung and pancreas, but barely detectable in kidney, thymus, colon and peripheral blood leukocytes.

  • 配列類似性

    Belongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.

  • ドメイン

    Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.

  • 細胞内局在

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • FLJ31113 antibody
      • IMA3_HUMAN antibody
      • Importin alpha 3 antibody
      • Importin alpha 4 subunit antibody
      • Importin alpha Q1 antibody
      • Importin subunit alpha 3 antibody
      • Importin subunit alpha-3 antibody
      • IPO A3 antibody
      • IPOA 3 antibody
      • IPOA3 antibody
      • Karyopherin alpha 4 (importin alpha 3) antibody
      • Karyopherin alpha 4 antibody
      • Karyopherin alpha 4 importin alpha 3 antibody
      • Karyopherin alpha 4 subunit antibody
      • Karyopherin subunit alpha 4 antibody
      • Karyopherin subunit alpha-4 antibody
      • KPNA 4 antibody
      • Kpna4 antibody
      • MGC12217 antibody
      • MGC26703 antibody
      • OTTHUMP00000213880 antibody
      • QIP 1 antibody
      • Qip1 antibody
      • Qip1 protein antibody
      • SRP 3 antibody
      • SRP3 antibody
      see all

    画像

    • Western blot - Anti-KPNA4 antibody [EPR26347-21] (AB302556)
      Western blot - Anti-KPNA4 antibody [EPR26347-21] (AB302556)
      All lanes : Anti-KPNA4 antibody [EPR26347-21] (ab302556) at 1/1000 dilution

      Lane 1 : 293T (human embryonic kidney epithelial cell) whole cell lysate
      Lane 2 : A549 (human lung carcinoma epithelial cell) whole cell lysate
      Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
      Lane 4 : L-929 (mouse connective tissue fibroblast) whole cell lysate
      Lane 5 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 7 : Raji (human Burkitt's lymphoma b lymphocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 57 kDa
      Observed band size: 58 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      Exposure time: Lanes 1-4: 81 seconds; Lanes 5-7: 59 seconds.

    • Western blot - Anti-KPNA4 antibody [EPR26347-21] (AB302556)
      Western blot - Anti-KPNA4 antibody [EPR26347-21] (AB302556)
      All lanes : Anti-KPNA4 antibody [EPR26347-21] (ab302556) at 1/1000 dilution

      Lane 1 : Human testis tissue lysate
      Lane 2 : Human kidney tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/4000 dilution

      Predicted band size: 57 kDa
      Observed band size: 58 kDa why is the actual band size different from the predicted?


      Exposure time: 59 seconds


      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      Low expression: Human kidney (PMID: 9395085). 

    • Western blot - Anti-KPNA4 antibody [EPR26347-21] (AB302556)
      Western blot - Anti-KPNA4 antibody [EPR26347-21] (AB302556)
      All lanes : Anti-GluN3A+GluN3B antibody [EPR25287-45] (ab302534) at 1/1000 dilution

      Lane 1 : Mouse testis tissue lysate
      Lane 2 : Mouse ovary tissue lysate
      Lane 3 : Rat testis tissue lysate
      Lane 4 : Rat ovary tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 57 kDa
      Observed band size: 58 kDa why is the actual band size different from the predicted?


      Exposure time: 81 seconds


      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA4 antibody [EPR26347-21] (AB302556)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA4 antibody [EPR26347-21] (AB302556)

      Immunohistochemical analysis of paraffin-embedded human lung tissue labeling KPNA4 with ab302556 at 1/5000 dilution (0.109 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Nuclear staining on human lung (PMID: 32320654). The section was incubated with ab302556 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA4 antibody [EPR26347-21] (AB302556)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA4 antibody [EPR26347-21] (AB302556)

      Immunohistochemical analysis of paraffin-embedded human testis tissue labeling KPNA4 with ab302556 at 1/5000 dilution (0.109 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Nuclear staining on human testis (PMID: 9395085). The section was incubated with ab302556 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA4 antibody [EPR26347-21] (AB302556)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA4 antibody [EPR26347-21] (AB302556)

      Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling KPNA4 with ab302556 at 1/5000 dilution (0.109 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Nuclear staining on human colon carcinoma (PMID: 31822798). The section was incubated with ab302556 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

    • Immunoprecipitation - Anti-KPNA4 antibody [EPR26347-21] (AB302556)
      Immunoprecipitation - Anti-KPNA4 antibody [EPR26347-21] (AB302556)

      KPNA4 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate with AB302556 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using AB302556 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate 10 µg (Inset)

      Lane 2: AB302556 IP in A549 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of AB302556 in A549 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 6 seconds

      Observed MW (kDa): 58. 

       

    • Anti-KPNA4 antibody [EPR26347-21] (AB302556)
      Anti-KPNA4 antibody [EPR26347-21] (AB302556)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302556 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302556 は論文での使用が確認できていません。

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