Anti-KPNA3 抗体 (ab6038)
Key features and details
- Goat polyclonal to KPNA3
- Suitable for: Flow Cyt, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-KPNA3 antibody
KPNA3 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to KPNA3 -
由来種
Goat -
アプリケーション
適用あり: Flow Cyt, ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Dog
-
免疫原
Synthetic peptide: DPTANLQTKEFNF, corresponding to C terminal amino acids 509-521 of Human KPNA3.
-
ポジティブ･コントロール
- WB: CaCo-2 cell lysate, MCF-7 cell lysate, HEK293 cell lysate. Flow Cyt: A431 cells ICC/IF: A431 cells
-
特記事項
GenBank Accession Number – NP_002258.The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
一次抗体 備考
The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6038の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use a concentration of 10 µg/ml.
|
|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
|
| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 58 kDa).
A 1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
Flow Cyt
Use a concentration of 10 µg/ml. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 58 kDa). A 1 hour primary incubation is recommended for this product. |
ターゲット情報
-
機能
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9. -
組織特異性
Ubiquitous. Highest levels in heart and skeletal muscle. -
配列類似性
Belongs to the importin alpha family.
Contains 10 ARM repeats.
Contains 1 IBB domain. -
ドメイン
Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 3839 Human
- Omim: 601892 Human
- SwissProt: O00505 Human
- Unigene: 527919 Human
-
別名
- SRP1 gamma antibody
- hSRP1 antibody
- IMA4_HUMAN antibody
see all
画像
-
Flow Cytometry - Anti-KPNA3 antibody (ab6038)Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
-
Western blot - Anti-KPNA3 antibody (ab6038)Lanes 1-2 : Anti-KPNA3 antibody (ab6038) at 0.03 µg/ml
Lane 3 : Anti-KPNA3 antibody (ab6038) at 0.01 µg/ml
Lane 1 : CaCo-2 cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : MCF-7 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 58 kDaDetected by chemiluminescence.
-
Immunocytochemistry/ Immunofluorescence - Anti-KPNA3 antibody (ab6038)Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (20)
ab6038 は 20 報の論文で使用されています。
- Xie Q et al. Domain in Fiber-2 interacted with KPNA3/4 significantly affects the replication and pathogenicity of the highly pathogenic FAdV-4. Virulence 12:754-765 (2021). PubMed: 33616472
- Thiele S et al. Cellular Importin-a3 Expression Dynamics in the Lung Regulate Antiviral Response Pathways against Influenza A Virus Infection. Cell Rep 31:107549 (2020). PubMed: 32320654
- Gao R et al. The selective NLRP3-inflammasome inhibitor MCC950 reduces myocardial fibrosis and improves cardiac remodeling in a mouse model of myocardial infarction. Int Immunopharmacol 74:105575 (2019). PubMed: 31299609
- Lyst MJ et al. Affinity for DNA Contributes to NLS Independent Nuclear Localization of MeCP2. Cell Rep 24:2213-2220 (2018). PubMed: 30157418
- Huang S et al. Pellet coculture of osteoarthritic chondrocytes and infrapatellar fat pad-derived mesenchymal stem cells with chitosan/hyaluronic acid nanoparticles promotes chondrogenic differentiation. Stem Cell Res Ther 8:264 (2017). PubMed: 29141683