Anti-KPNA2 抗体 (ab70160)
Key features and details
- Rabbit polyclonal to KPNA2
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-KPNA2 antibody
KPNA2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KPNA2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chimpanzee, Orangutan
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免疫原
A region between residue 475 and the C-terminus (residue 529) of human KPNA2 (NP_002257.1).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70160の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000 - 1/25000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
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| IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use a concentration of 0.04 µg/ml.
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| 特記事項 |
|---|
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WB
1/10000 - 1/25000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use a concentration of 0.04 µg/ml. |
ターゲット情報
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機能
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. -
組織特異性
Expressed ubiquitously. -
配列類似性
Belongs to the importin alpha family.
Contains 10 ARM repeats.
Contains 1 IBB domain. -
ドメイン
Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3838 Human
- Entrez Gene: 16647 Mouse
- Omim: 600685 Human
- SwissProt: P52292 Human
- SwissProt: P52293 Mouse
- Unigene: 594238 Human
- Unigene: 12508 Mouse
- Unigene: 391577 Mouse
see all -
別名
- IMA1_HUMAN antibody
- Importin alpha 1 antibody
- Importin alpha 2 antibody
see all
画像
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Western blot - Anti-KPNA2 antibody (ab70160)All lanes : Anti-KPNA2 antibody (ab70160) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg
Predicted band size: 58 kDa -
Immunoprecipitation - Anti-KPNA2 antibody (ab70160)Immunoprecipitation of KPNA2 with ab70160 at 0.04 µg/mL.
Lane 1 and 2: Different lots of ab70160 + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293 cells prepared using NETN lysis buffer.Lane 2: IgG control + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293 cells prepared using NETN lysis buffer.
Detection: Chemiluminescence
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA2 antibody (ab70160)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling KPNA2 with ab70160 at 1/1000 (1µg/ml; left and right) and 1/5000 (0.2µg/ml; middle). Detection: DAB.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab70160 は 9 報の論文で使用されています。
- Wang W et al. Karyopherin α deficiency contributes to human preimplantation embryo arrest. J Clin Invest 133:N/A (2023). PubMed: 36647821
- Wei Z et al. SMARCC1 Enters the Nucleus via KPNA2 and Plays an Oncogenic Role in Bladder Cancer. Front Mol Biosci 9:902220 (2022). PubMed: 35669562
- Wu JW et al. Spatiotemporal gating of Stat nuclear influx by Drosophila Npas4 in collective cell migration. Sci Adv 8:eabm2411 (2022). PubMed: 35867785
- Zeng F et al. KPNA2 interaction with CBX8 contributes to the development and progression of bladder cancer by mediating the PRDM1/c-FOS pathway. J Transl Med 19:112 (2021). PubMed: 33731128
- Frottin F et al. Multiple pathways of toxicity induced by C9orf72 dipeptide repeat aggregates and G4C2 RNA in a cellular model. Elife 10:N/A (2021). PubMed: 34161229
- Pallett MA et al. Vaccinia Virus BBK E3 Ligase Adaptor A55 Targets Importin-Dependent NF-?B Activation and Inhibits CD8+ T-Cell Memory. J Virol 93:N/A (2019). PubMed: 30814284
- Woerner AC et al. Cytoplasmic protein aggregates interfere with nucleocytoplasmic transport of protein and RNA. Science 351:173-6 (2016). PubMed: 26634439
- Lee KH et al. C9orf72 Dipeptide Repeats Impair the Assembly, Dynamics, and Function of Membrane-Less Organelles. Cell 167:774-788.e17 (2016). PubMed: 27768896
- Carter DM et al. Proteomic identification of nuclear processes manipulated by cytomegalovirus early during infection. Proteomics 15:1995-2005 (2015). PubMed: 25758553