Anti-KMT6/EZH2 (phospho T487) 抗体 [EPR24903-104] (BSA and Azide free) (ab300568)

This data was developed using ab300567, the same antibody clone in a different buffer formulation.

Blocking / Diluting buffer and concentration:5% NFDM/TBST

This data was developed using ab300567, the same antibody clone in a different buffer formulation.

Blocking / Diluting buffer and concentration: 5% NFDM/TBST

This data was developed using ab300567, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat colon labeling KMT6/EZH2 (phospho Thr487) with ab300567 at 1/2000 dilution (0.221 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Nuclear staining on rat colon without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab300567 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using ab300567, the same antibody clone in a different buffer formulation.

Concentration of ab300567: 1/1000 dilution (0.441 μg/ml)

Secondary ab: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/100000 dilution

Blocking/diluting buffer and concentration: 5% NFDM/TBST

Lane 1: KMT6/EZH2 (phospho Thr487) peptide a

Lane 2: KMT6/EZH2 (phospho Thr487) peptide b

Lane 3: KMT6/EZH2 non-phospho peptide corresponding to the region of phospho peptides a and b

Exposure time: 3 minutes

This data was developed using ab300567, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse spleen labeling KMT6/EZH2 (phospho Thr487) with ab300567 at 1/2000 dilution (0.221 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Nuclear staining on mouse spleen without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab300567 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using ab300567, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsil labeling KMT6/EZH2 (phospho Thr487) with ab300567 at 1/2000 dilution (0.221 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Nuclear staining on human tonsil without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab300567 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using ab300567, the same antibody clone in a different buffer formulation.

Flow Cytometry (Intracellular) analysis of HeLa (human cervix adenocarcinoma epithelial cells) labeling KMT6/EZH2(phos Thr487) (Right) with ab300567 at a 1/500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, (ab150081)) was used as the secondary antibody at a 1/5000 dilution. Rabbit monoclonal IgG (ab172730) was used as an isotype control (Left).

This data was developed using ab300567, the same antibody clone in a different buffer formulation.

Flow Cytometry (Intracellular) analysis of HeLa (human cervix adenocarcinoma epithelial cell) treated with 20μM nocodazole for 24 hours (Right) / Untreated control (Left) labeling KMT6/EZH2(phos Thr487) with ab300567 at a 1/500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, (ab150081)) was used as the secondary antibody at a 1/5000 dilution.