Anti-KMT6/EZH2 (phospho T487) 抗体 [EPR24903-104] (ab300567)

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Western blot - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (AB300567)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR24903-104] to KMT6 / EZH2 (phospho T487)
Suitable for: WB, Dot blot, IHC-P, Flow Cyt (Intra)
Reacts with: Mouse, Rat, Human

Carrier Free

製品名

Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104]
KMT6 / EZH2 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR24903-104] to KMT6 / EZH2 (phospho T487)
由来種

Rabbit
アプリケーション

適用あり: WB, Dot blot, IHC-P, Flow Cyt (Intra)more details
適用なし: ICC/IF or IP
種交差性

交差種: Mouse, Rat, Human
免疫原

This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: HeLa treated and untreated with 0.5µM nocodazole; HeLa treated and untreated with 2mM thymidine; Dot Blot: KMT6/EZH2(phospho Thr487) peptide IHC-P: Human tonsil, mouse spleen, and rat colon FFPE tissue sections Flow Cyt (Intra): HeLa (human cervix adenocarcinoma epithelial cells); HeLa treated and untreated with 20µM nocodazole.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR24903-104
アイソタイプ

IgG
研究分野
- Cancer
- Tumor biomarkers
- Enzymes
- Other

Alternative Versions
- Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (BSA and Azide free) (ab300568)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab300567の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB		1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa).
Dot blot		1/1000.
IHC-P		1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)		1/500.

特記事項
WB 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa).
Dot blot 1/1000.
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra) 1/500.

追加情報

Is unsuitable for ICC/IF or IP.

機能

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes.
組織特異性

Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
配列類似性

Belongs to the histone-lysine methyltransferase family. EZ subfamily.
Contains 1 SET domain.
発生段階

Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary.
翻訳後修飾

Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity.
細胞内局在

Nucleus.
Target information above from: UniProt accession Q15910 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 2146 Human
- Entrez Gene: 14056 Mouse
- Entrez Gene: 312299 Rat
- Omim: 601573 Human
- SwissProt: Q15910 Human
- SwissProt: Q61188 Mouse
- Unigene: 444082 Human
- Unigene: 246688 Mouse
別名
- Enhancer of zeste 2 antibody
- enhancer of zeste 2 polycomb repressive complex 2 subunit antibody
- Enhancer of zeste homolog 2 (Drosophila) antibody
- Enhancer of zeste homolog 2 antibody
- Enhancer of zeste, Drosophila, homolog 2 antibody
- ENX 1 antibody
- Enx 1h antibody
- ENX-1 antibody
- ENX1 antibody
- Enx1h antibody
- EZH 2 antibody
- EZH1 antibody
- EZH2 antibody
- EZH2_HUMAN antibody
- EZH2b antibody
- Histone-lysine N-methyltransferase EZH2 antibody
- KMT 6 antibody
- KMT6 antibody
- KMT6A antibody
- Lysine N-methyltransferase 6 antibody
- MGC9169 antibody
- WVS antibody
- WVS2 antibody
see all

Western blot - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (AB300567)

All lanes : Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (ab300567) at 1/1000 dilution

Lane 1 : HeLa treated with 2 mM thymidine for 16 hours, and then 10 nM nocodazole for 24 hours, whole cell lysate (untreated membrane)
Lane 2 : HeLa treated with 2 mM thymidine for 16 hours, and then 10 nM nocodazole for 24 hours, whole cell lysate (phosphatase treated membrane)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

Predicted band size: 85 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?

Exposure time: 26 seconds

Blocking / Diluting buffer and concentration:5% NFDM/TBST
Western blot - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (AB300567)

All lanes : Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (ab300567) at 1/5000 dilution

Lane 1 : Untreated HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HeLa treated with 0.5 µM nocodazole for 24 hours, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 85 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?

Exposure time: 70 seconds

Blocking / Diluting buffer and concentration: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (AB300567)

Immunohistochemical analysis of paraffin-embedded rat colon labeling KMT6/EZH2 (phospho Thr487) with ab300567 at 1/2000 dilution (0.221 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Nuclear staining on rat colon without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab300567 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (AB300567)

Immunohistochemical analysis of paraffin-embedded mouse spleen labeling KMT6/EZH2 (phospho Thr487) with ab300567 at 1/2000 dilution (0.221 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Nuclear staining on mouse spleen without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab300567 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (AB300567)

Immunohistochemical analysis of paraffin-embedded human tonsil labeling KMT6/EZH2 (phospho Thr487) with ab300567 at 1/2000 dilution (0.221 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Nuclear staining on human tonsil without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab300567 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Flow Cytometry (Intracellular) - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (AB300567)

Flow Cytometry (Intracellular) analysis of HeLa (human cervix adenocarcinoma epithelial cells) labeling KMT6/EZH2 (phospho Thr487) (Right) with ab300567 at a 1/500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, (ab150081)) was used as the secondary antibody at a 1/5000 dilution. Rabbit monoclonal IgG (ab172730) was used as an isotype control (Left).
Flow Cytometry (Intracellular) - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (AB300567)

Flow Cytometry (Intracellular) analysis of HeLa (human cervix adenocarcinoma epithelial cell) treated with 20 μM nocodazole for 24 hours (Right) / Untreated control (Left) labeling KMT6/EZH2 (phospho Thr487) with ab300567 at a 1/500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, (ab150081)) was used as the secondary antibody at a 1/5000 dilution.
Dot Blot - Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (AB300567)

Concentration of ab300567: 1/1000 dilution (0.441 μg/ml)

Secondary ab: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/100000 dilution

Blocking/diluting buffer and concentration: 5% NFDM/TBST

Lane 1: KMT6/EZH2 (phospho Thr487) peptide a

Lane 2: KMT6/EZH2 (phospho Thr487) peptide b

Lane 3: KMT6/EZH2 non-phospho peptide corresponding to the region of phospho peptides a and b

Exposure time: 3 minutes
Anti-KMT6/EZH2 (phospho T487) antibody [EPR24903-104] (AB300567)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

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ab300567 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300567 は 1 報の論文で使用されています。

Song D et al. Single-cell RNA sequencing reveals the heterogeneity of epithelial cell and fibroblast cells from non- to metastatic lymph node OTSCC. FASEB J 38:e23390 (2024). PubMed: 38169064

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

発生段階

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (1)

レビューと Q&A