Anti-KMT6 / EZH2 抗体 (ab186006)
Key features and details
- Rabbit polyclonal to KMT6 / EZH2
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-KMT6 / EZH2 antibody
KMT6 / EZH2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KMT6 / EZH2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Xenopus tropicalis
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免疫原
Synthetic peptide within Human KMT6/ EZH2 aa 650 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. CAA64955.1
Database link: Q15910 -
ポジティブ･コントロール
- 293T, HeLa, Jurkat, and Mouse TCMK-1 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186006の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/500 - 1/2500. Predicted molecular weight: 85 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
1/500 - 1/2500. Predicted molecular weight: 85 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. -
組織特異性
Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis. -
配列類似性
Belongs to the histone-lysine methyltransferase family. EZ subfamily.
Contains 1 SET domain. -
発生段階
Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary. -
翻訳後修飾
Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 509106 Cow
- Entrez Gene: 475511 Dog
- Entrez Gene: 2146 Human
- Entrez Gene: 14056 Mouse
- Entrez Gene: 100625497 Pig
- Entrez Gene: 100345157 Rabbit
- Entrez Gene: 709075 Rhesus monkey
- Entrez Gene: 100381148 Xenopus laevis
see all -
別名
- Enhancer of zeste 2 antibody
- enhancer of zeste 2 polycomb repressive complex 2 subunit antibody
- Enhancer of zeste homolog 2 (Drosophila) antibody
see all
画像
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Western blot - Anti-KMT6 / EZH2 antibody (ab186006)Lane 1 : Anti-KMT6 / EZH2 antibody (ab186006) at 1 µg
Lanes 2-4 : Anti-KMT6 / EZH2 antibody (ab186006) at 1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : Mouse TCMK-1 cells.lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 85 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 / EZH2 antibody (ab186006)Mouse plasmacytoma tissue stained with ab186006 at a diltution of 1:1000 to detect EZH2.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 / EZH2 antibody (ab186006)Human ovarian tissue stained with ab186006 at a diltution of 1:1000 to detect EZH2.
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Immunoprecipitation - Anti-KMT6 / EZH2 antibody (ab186006)Detection of KMT6 / EZH2 by Western Blot of Immunprecipitate.
Lane 1: ab186006 at 1 µg/ml labeling KMT6 / EZH2 in 293T whole cell lysate immunoprecipitated using ab186006 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with an exposure time of 3 minutes.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (82)
ab186006 は 82 報の論文で使用されています。
- Wu JC et al. ONC201 Suppresses Neuroblastoma Growth by Interrupting Mitochondrial Function and Reactivating Nuclear ATRX Expression While Decreasing MYCN. Int J Mol Sci 24:N/A (2023). PubMed: 36675163
- Song BF et al. MiR-124-3p inhibits tumor progression in prostate cancer by targeting EZH2. Funct Integr Genomics 23:80 (2023). PubMed: 36884182
- Sbirkov Y et al. Dual inhibition of EZH2 and G9A/GLP histone methyltransferases by HKMTI-1-005 promotes differentiation of acute myeloid leukemia cells. Front Cell Dev Biol 11:1076458 (2023). PubMed: 37035245
- Paules EM et al. Choline Regulates SOX4 through miR-129-5p and Modifies H3K27me3 in the Developing Cortex. Nutrients 15:N/A (2023). PubMed: 37375678
- Mao Q et al. CRISPR/Cas9‑mediated EZH2 knockout suppresses the proliferation and migration of triple‑negative breast cancer cells. Oncol Lett 26:343 (2023). PubMed: 37427349