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AB222145

Anti-KMT3B / NSD1 抗体

Anti-KMT3B / NSD1 antibody

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(2 Publications)

Rabbit Polyclonal KMT3B / NSD1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NSD1 aa 800 to C-terminus.

別名を表示する

ARA267, KMT3B, NSD1, Androgen receptor coactivator 267 kDa protein, Androgen receptor-associated protein of 267 kDa, H3-K36-HMTase, Lysine N-methyltransferase 3B, Nuclear receptor-binding SET domain-containing protein 1, NR-binding SET domain-containing protein

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-KMT3B / NSD1 antibody (AB222145)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KMT3B / NSD1 antibody (AB222145)

PFA fixed/ Triton X-100 permeabilized HEK293 (human epithelial cell line from embryonic kidney) cells stained for KMT3B / NSD1 (green) using ab222145 (4 μg/ml) in Immunocytochemistry/ Immunofluorescence.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human NSD1 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96L73

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

KMT3B also known as NSD1 is a histone methyltransferase enzyme with a significant role in chromatin modification. This protein adds methyl groups to histone H3 at lysine 36 (H3K36). With a molecular mass around 280 kDa NSD1 is mostly expressed in tissues such as the brain and skeletal muscle. It plays an integral role in controlling gene expression and maintaining genomic stability through its actions on chromatin.
Biological function summary

NSD1 modulates transcription regulation by interacting with other nuclear proteins. As a part of the epigenetic regulation mechanism it is involved in complex processes of genome organization. By methylating H3K36 NSD1 affects chromatin dynamics and influences transcriptional activation and repression. NSD1 supports the orchestration of transcriptional changes in response to cellular signals and environmental stimuli.

Pathways

NSD1 interfaces with transcriptional regulation and chromatin remodeling pathways. Its enzymatic action on H3K36 links NSD1 to pathways governing cell growth and differentiation. Through its interactions NSD1 associates with proteins like MLL and SETD2 highlighting its role in the regulation of gene expression and cellular developmental pathways.

NSD1's dysfunction associates with Sotos syndrome and acute myeloid leukemia. Mutations in NSD1 lead to overgrowth and developmental delay characteristics observed in Sotos syndrome. In leukemia disrupted methylation patterns arise from NSD1 alterations affecting proteins like NUP98 contributing to oncogenic transformation and malignancy progression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Histone methyltransferase that dimethylates Lys-36 of histone H3 (H3K36me2). Transcriptional intermediary factor capable of both negatively or positively influencing transcription, depending on the cellular context.
See full target information NSD1
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109164 PubMed38414856

2024

Optic atrophy 1 mediates muscle differentiation by promoting a metabolic switch via the supercomplex assembly factor SCAF1.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Triolo,Nicole Baker,Soniya Agarwal,Nikita Larionov,Tina Podinić,Mireille Khacho

Frontiers in oncology 11:808291 PubMed35127520

2022

KB-68A7.1 Inhibits Hepatocellular Carcinoma Development Through Binding to NSD1 and Suppressing Wnt/β-Catenin Signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhua Zhang,Jianqun Xu,Huan Cao,Mi Jiang,Jun Xiong
View all publications
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