Anti-KMT3A / HYPB / HIF-1 抗体 (ab184190)
Key features and details
- Rabbit polyclonal to KMT3A / HYPB / HIF-1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-KMT3A / HYPB / HIF-1 antibody
KMT3A / HYPB / HIF-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KMT3A / HYPB / HIF-1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment within Human KMT3A/ HYPB/ HIF-1 aa 453-679. The exact sequence is proprietary.
Database link: Q9BYW2 -
ポジティブ･コントロール
- Raji and K562 whole cell lysates; HBL435 xenograft tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184190の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 288 kDa.
|
|
| IHC-P |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 288 kDa. |
|
IHC-P
1/100 - 1/1000. |
ターゲット情報
-
機能
Histone methyltransferase that methylates 'Lys-36' of histone H3. H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation. Probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. Binds DNA at promoters. May also act as a transcription activator that binds to promoters. Binds to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the histone-lysine methyltransferase family. SET2 subfamily.
Contains 1 AWS domain.
Contains 1 post-SET domain.
Contains 1 SET domain.
Contains 1 WW domain. -
ドメイン
The low charge region mediates the transcriptional activation activity. -
翻訳後修飾
May be automethylated. -
細胞内局在
Nucleus. Chromosome. - Information by UniProt
-
参照データベース
- Entrez Gene: 29072 Human
- Omim: 612778 Human
- SwissProt: Q9BYW2 Human
- Unigene: 517941 Human
-
別名
- EC 2.1.1.43 antibody
- FLJ16420 antibody
- FLJ22472 antibody
see all
画像
-
Western blot - Anti-KMT3A / HYPB / HIF-1 antibody (ab184190)All lanes : Anti-KMT3A / HYPB / HIF-1 antibody (ab184190) at 1/1000 dilution
Lane 1 : Raji whole cell lysate
Lane 2 : K562 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 288 kDa5% SDS PAGE
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT3A / HYPB / HIF-1 antibody (ab184190)Immunohistochemical analysis of paraffin-embedded HBL435 xenograft tissue, labeling KMT3A / HYPB / HIF-1 with ab184190 at 1/500 dilution.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab184190 は 2 報の論文で使用されています。
- Zou G et al. Long non-coding RNA BRE-AS1 inhibits proliferation, migration and invasion of clear cell renal cell carcinoma by downregulating miR-106b-5p. Histol Histopathol 39:729-737 (2024). PubMed: 37530129
- Liu L et al. Loss of SETD2, but not H3K36me3, correlates with aggressive clinicopathological features of clear cell renal cell carcinoma patients. Biosci Trends 11:214-220 (2017). PubMed: 28260718