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Anti-KMT2B / MLL4 抗体 [4C10] (ab56770)

Reviews (1) Submit a question References (1)
Western blot - Anti-KMT2B / MLL4 antibody [4C10] (ab56770)
  • Flow Cytometry - Anti-KMT2B / MLL4 antibody [4C10] (ab56770)

Key features and details

  • Mouse monoclonal [4C10] to KMT2B / MLL4
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-KMT2B / MLL4 antibody [4C10]
    KMT2B / MLL4 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [4C10] to KMT2B / MLL4

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, Flow Cytmore details

  • 種交差性

    交差種: Human, Recombinant fragment

  • 免疫原

    Recombinant fragment (GST-tag) corresponding to Human KMT2B/ MLL4 aa 800-950. Entrez gene ID: 9757
    Database link: Q9UMN6

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 11th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab56770の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
Use at an assay dependent concentration.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
特記事項
WB
Use at an assay dependent concentration.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Histone methyltransferase. Methylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2. Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development. Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation.

  • 組織特異性

    Widely expressed. Highest levels in testis. Also found in brain, bone marrow, heart, muscle, kidney, placenta, spleen, thymus, prostate, ovary, intestine, colon, peripheral blood lymphocytes and pancreas. Often amplified in pancreatic carcinomas.

  • 配列類似性

    Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX/MLL subfamily.
    Contains 3 A.T hook DNA-binding domains.
    Contains 1 CXXC-type zinc finger.
    Contains 1 FYR C-terminal domain.
    Contains 1 FYR N-terminal domain.
    Contains 3 PHD-type zinc fingers.
    Contains 1 post-SET domain.
    Contains 1 SET domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Histone-lysine N-methyltransferase 2B antibody
      • HRX2 antibody
      • KIAA0304 gene product antibody
      • KMT2B antibody
      • KMT2B_HUMAN antibody
      • Likely ortholog of mouse WW domain binding protein 7 antibody
      • Lysine N-methyltransferase 2B antibody
      • mixed lineage leukemia 4 antibody
      • Mixed lineage leukemia gene homolog 2 protein antibody
      • MLL2 antibody
      • myeloid lymphoid leukemia 4 antibody
      • Myeloid lymphoid or mixed lineage leukemia protein 4 antibody
      • Myeloid/lymphoid or mixed-lineage leukemia protein 4 antibody
      • Trithorax homolog 2 antibody
      • trithorax homologue 2 antibody
      • TRX2 antibody
      • WBP-7 antibody
      • WBP7 antibody
      • WW domain-binding protein 7 antibody
      see all

    画像

    • Western blot - Anti-KMT2B / MLL4 antibody [4C10] (ab56770)
      Western blot - Anti-KMT2B / MLL4 antibody [4C10] (ab56770)

      Western blot against tagged recombinant protein immunogen using ab56770 MLL4 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa

      This image was generated using the ascites version of the product.

    • Flow Cytometry - Anti-KMT2B / MLL4 antibody [4C10] (ab56770)
      Flow Cytometry - Anti-KMT2B / MLL4 antibody [4C10] (ab56770)

      Overlay histogram showing HL60 cells stained with ab56770 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56770, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

      This image was generated using the ascites version of the product.

    参考文献 (1)

    ab56770 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab56770 は 1 報の論文で使用されています。

    • Zhao G  et al. M2-like tumor-associated macrophages transmit exosomal miR-27b-3p and maintain glioblastoma stem-like cell properties. Cell Death Discov 8:350 (2022). PubMed: 35927251

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-KMT2B / MLL4 antibody [4C10]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (Liver cell lines)
    Gel Running Conditions
    Reduced Denaturing (6%)
    Loading amount
    30 µg
    Specification
    Liver cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 01 2020

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