AB243867

Anti-KMT2A / MLL 抗体 [BL-175-7E8]

Anti-KMT2A / MLL antibody [BL-175-7E8]

Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal KMT2A / MLL antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human KMT2A aa 700-800.

別名を表示する

ALL1, CXXC7, HRX, HTRX, MLL, MLL1, TRX1, KMT2A, Histone-lysine N-methyltransferase 2A, Lysine N-methyltransferase 2A, ALL-1, CXXC-type zinc finger protein 7, Cysteine methyltransferase KMT2A, Myeloid/lymphoid or mixed-lineage leukemia, Myeloid/lymphoid or mixed-lineage leukemia protein 1, Trithorax-like protein, Zinc finger protein HRX

2 Images

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Immunoprecipitation - Anti-KMT2A / MLL antibody [BL-175-7E8] (AB243867)

KMT2A / MLL was immunoprecipitated from 1 mg HEK-293T whole cell lysate with ab243867 at 10 µl per reaction Western blot was performed on the immunoprecipitate using ab243867 at 1/1000 dilution. Lane 1 : ab243867 IP in HEK-293T cell lysate.

Lane 2 : Contol IgG in HEK-293T cell lysate.

Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-KMT2A / MLL antibody [BL-175-7E8] (ab243867)

Predicted band size: 432 kDa

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Western blot - Anti-KMT2A / MLL antibody [BL-175-7E8] (AB243867)

Western blot analysis using ab243867 at 1/1000 dilution.

Lane 1 : HEK-293T whole cell lysate (50 µg).

Lane 2 : HeLa whole cell lysate (50 µg).

Lane3 : MCF7 whole cell lysate (50 µg).

Lane 4 : HepG2 whole cell lysate (50 µg).

Lane 5 : A549 whole cell lysate (50 µg).

Lane 6 : SW620 whole cell lysate (50 µg).

a HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection : chemiluminescence with an exposure time of 3 minutes.

All lanes:

Western blot - Anti-KMT2A / MLL antibody [BL-175-7E8] (ab243867)

Predicted band size: 432 kDa

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Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

BL-175-7E8

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, WB

applications

免疫原

Synthetic Peptide within Human KMT2A aa 700-800. The exact immunogen used to generate this antibody is proprietary information.

Q03164

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Use 10μl/0.5mg lysate.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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製品の詳細

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製に関する特記事項

Recombinant antibody was purified from cell culture supernatant.

バッファー組成

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The KMT2A protein also known as MLL (Mixed-Lineage Leukemia) is a large protein with a molecular weight of approximately 431 kDa. This protein functions as a histone methyltransferase which means it adds methyl groups to histone proteins specifically at the lysine 4 position on histone H3 (H3K4). This modification impacts chromatin structure and gene expression. KMT2A/MLL is chiefly expressed in hematopoietic stem cells and various tissue types indicating its broad role in regulation across the body. The protein features a SET domain a characteristic motif for proteins involved in chromatin modification.

Biological function summary

This methyltransferase plays a significant role in regulating gene expression required for normal hematopoietic development and maintenance. KMT2A/MLL forms part of the multi-protein complex called COMPASS-like complex which is essential for its function in methylation activity. Within this complex it associates with other proteins like WDR5 RBBP5 and ASH2L that collaborate to control transcriptional elongation. KMT2A/MLL is also involved in maintaining the expression of various homeobox (HOX) genes which are critical for embryonic development and cell differentiation.

Pathways

KMT2A/MLL plays an integral role in hematopoietic and developmental signaling pathways. One key pathway is the Wnt signaling pathway important for regulating stem cell pluripotency and cell fate decisions. Another pathway is the Notch signaling pathway important for cell differentiation processes. KMT2A interacts with proteins within these pathways such as CXXC1 which links it to the DNA binding properties required for regulating target gene expression.

KMT2A/MLL is closely linked to leukemia notably acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML). A significant factor contributing to these associations is the gene fusions involving KMT2A often created during chromosomal translocations which result in oncogenic activation. In these leukemias it can form fusion proteins like MLL-AF4 impacting the normal regulatory activities of KMT2A. This aberrant interaction with proteins such as MEN1 can contribute to oncogenic processes by altering transcription and leading to uncontrolled cell growth.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Histone methyltransferase that plays an essential role in early development and hematopoiesis (PubMed : 12453419, PubMed : 15960975, PubMed : 19187761, PubMed : 19556245, PubMed : 20677832, PubMed : 21220120, PubMed : 26886794). Catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac) (PubMed : 12453419, PubMed : 15960975, PubMed : 19187761, PubMed : 19556245, PubMed : 20677832, PubMed : 21220120, PubMed : 24235145, PubMed : 26886794). Catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism. Part of chromatin remodeling machinery predominantly forms H3K4me1 and H3K4me2 methylation marks at active chromatin sites where transcription and DNA repair take place (PubMed : 12453419, PubMed : 15960975, PubMed : 19187761, PubMed : 19556245, PubMed : 20677832, PubMed : 21220120, PubMed : 25561738, PubMed : 26886794). Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity (PubMed : 19187761, PubMed : 26886794). Has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', while it has higher activity toward H3 acetylated on 'Lys-9' (PubMed : 19187761). Binds to unmethylated CpG elements in the promoter of target genes and helps maintain them in the nonmethylated state (PubMed : 20010842). Required for transcriptional activation of HOXA9 (PubMed : 12453419, PubMed : 20010842, PubMed : 20677832). Promotes PPP1R15A-induced apoptosis (PubMed : 10490642). Plays a critical role in the control of circadian gene expression and is essential for the transcriptional activation mediated by the CLOCK-BMAL1 heterodimer (By similarity). Establishes a permissive chromatin state for circadian transcription by mediating a rhythmic methylation of 'Lys-4' of histone H3 (H3K4me) and this histone modification directs the circadian acetylation at H3K9 and H3K14 allowing the recruitment of CLOCK-BMAL1 to chromatin (By similarity). Also has auto-methylation activity on Cys-3882 in absence of histone H3 substrate (PubMed : 24235145).

See full target information KMT2A

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1011 PubMed40617868

2025

TTK activates ATR through RPA2 phosphorylation to promote olaparib resistance in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Gonghua Qi,Hanlin Ma,Jingying Chen,Panpan Gai,Kai Teng

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KMT2A / MLL 抗体 [BL-175-7E8]

Anti-KMT2A / MLL antibody [BL-175-7E8]

Immunoprecipitation - Anti-KMT2A / MLL antibody [BL-175-7E8] (AB243867)

Western blot - Anti-KMT2A / MLL antibody [BL-175-7E8] (AB243867)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise