Anti-KMT1E/SETDB1 抗体 [EPR24218-43] (BSA and Azide free) (ab300574)

This data was developed using ab300573, the same antibody clone in a different buffer formulation. 

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Note: Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

The identity of the lower MW band at approximately 70kDa is unknown.

Exposure time: 48 seconds

This data was developed using ab300573, the same antibody clone in a different buffer formulation. 

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Note: Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

Exposure time: 48 seconds

This data was developed using 300573, the same antibody clone in a different buffer formulation. 

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Note: Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

Exposure time: 48 seconds

This data was developed using ab300573, the same antibody clone in a different buffer formulation.

KMT1E / SETDB1 was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate 10 ug with ab300573 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300573 at 1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate 10 ug

Lane 2: ab300573 IP in K562 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300573 in K562 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 128 seconds

Lysate was freshly made and used for Immunoprecipitation immediately to minimize protein degradation.The band beneath the target band (100 kDa) is likely to be degraded target fragments.