Anti-KMT1E / SETDB1 抗体 [5H6A12] (ab107225)
![Western blot - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225)](https://www.abcam.co.jp/ps/products/107/ab107225/Images/ab107225-119669-anti-kmt1e-setdb1-antibody-5h6a12-western-blot.jpg "Western blot - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225)")
Key features and details
- Mouse monoclonal [5H6A12] to KMT1E / SETDB1
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG1
製品の概要
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製品名
Anti-KMT1E / SETDB1 antibody [5H6A12]
KMT1E / SETDB1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5H6A12] to KMT1E / SETDB1 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human, African green monkey -
免疫原
Purified recombinant fragment of human SETDB1 expressed in E. Coli.
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ポジティブ･コントロール
- MCF-7,T47D, HEK293, JURKAT, NIH/3T3, F9, RAW246.7 and COS-7 cell lysates.
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特記事項
This product was changed from ascites to supernatant. Lot no’s high than GR223392-13 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
5H6A12 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab107225の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 180 kDa.
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| ICC/IF | (1) |
1/200 - 1/1000.
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| 特記事項 |
|---|
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Flow Cyt
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB
1/500 - 1/2000. Predicted molecular weight: 180 kDa. |
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ICC/IF
1/200 - 1/1000. |
ターゲット情報
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機能
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. -
組織特異性
Widely expressed. High expression in testis. -
配列類似性
Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 1 MBD (methyl-CpG-binding) domain.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.
Contains 2 Tudor domains. -
ドメイン
The pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
Isoform 2 lacks all domains required for histone methyltransferase activity. -
細胞内局在
Nucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin. - Information by UniProt
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参照データベース
- Entrez Gene: 9869 Human
- Entrez Gene: 84505 Mouse
- Omim: 604396 Human
- SwissProt: Q15047 Human
- SwissProt: O88974 Mouse
- Unigene: 643565 Human
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別名
- AU022152 antibody
- EC 2.1.1.43 antibody
- ERG-associated protein with SET domain antibody
see all
画像
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Western blot - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225)All lanes : Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225) at 1/500 dilution
Lane 1 : MCF-7
Lane 2 : T47D
Lane 3 : HEK293
Lane 4 : JURKAT
Lane 5 : NIH/3T3
Lane 6 : F9
Lane 7 : RAW264.7
Lane 8 : COS-7
Predicted band size: 180 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225)](https://www.abcam.co.jp/ps/products/107/ab107225/Images/ab107225-119668-anti-kmt1e-setdb1-antibody-5h6a12-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225)Immunofluoresence detection of SETDB1 in LOVO cells using ab107225 (green). Actin fillaments have been labeled with Alexa Fluor-555 phalloidin (red). -
![Flow Cytometry - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225)](https://www.abcam.co.jp/ps/products/107/ab107225/Images/ab107225-119667-anti-kmt1e-setdb1-antibody-5h6a12-flow-cytometry.jpg)
Flow Cytometry - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225)Overlay histogram showing Ramos cells stained with ab107225 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab107225, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab107225 は 8 報の論文で使用されています。
- Carraro M et al. DAXX adds a de novo H3.3K9me3 deposition pathway to the histone chaperone network. Mol Cell 83:1075-1092.e9 (2023). PubMed: 36868228
- Rapone R et al. The cytoplasmic fraction of the histone lysine methyltransferase Setdb1 is essential for embryonic stem cells. iScience 26:107386 (2023). PubMed: 37559904
- Cheng EC et al. The Essential Function of SETDB1 in Homologous Chromosome Pairing and Synapsis during Meiosis. Cell Rep 34:108575 (2021). PubMed: 33406415
- Fatima A et al. The ubiquitin-conjugating enzyme UBE2K determines neurogenic potential through histone H3 in human embryonic stem cells. Commun Biol 3:262 (2020). PubMed: 32451438
- Bilen M et al. Methionine mediates resilience to chronic social defeat stress by epigenetic regulation of NMDA receptor subunit expression. Psychopharmacology (Berl) 237:3007-3020 (2020). PubMed: 32564114
- Adoue V et al. The Histone Methyltransferase SETDB1 Controls T Helper Cell Lineage Integrity by Repressing Endogenous Retroviruses. Immunity 50:629-644.e8 (2019). PubMed: 30737147
- Ropa J et al. PAF1 complex interactions with SETDB1 mediate promoter H3K9 methylation and transcriptional repression of Hoxa9 and Meis1 in acute myeloid leukemia. Oncotarget 9:22123-22136 (2018). PubMed: 29774127
- Huang J et al. Enhanced expression of SETDB1 possesses prognostic value and promotes cell proliferation, migration and invasion in nasopharyngeal carcinoma. Oncol Rep 40:1017-1025 (2018). PubMed: 29901162