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リコンビナントRabMAb

Anti-Klotho 抗体 [EPR6856] (ab181373)

Reviews (1) Submit a question References (30)
Western blot - Anti-Klotho antibody [EPR6856] (ab181373)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody [EPR6856] (ab181373)
  • Western blot - Anti-Klotho antibody [EPR6856] (ab181373)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody [EPR6856] (ab181373)
  • Western blot - Anti-Klotho antibody [EPR6856] (ab181373)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody [EPR6856] (ab181373)
  • Anti-Klotho antibody [EPR6856] (ab181373)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6856] to Klotho
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

製品の概要

  • 製品名

    Anti-Klotho antibody [EPR6856]
    Klotho 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR6856] to Klotho

  • 由来種

    Rabbit

  • 特異性

    When we tested the antibody in human samples, the only form of the protein that we are able to detect is the secreted one (about 65kDa). When we tested the antibody in mouse samples (wt vs KO) we could only detect the membrane bound form of the protein (about 130kDa). This difference could be due to the nature of the samples (different species). However, we do not have a clear explanation about this. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented here.

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: ICC/IF

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Synthetic peptide within Human Klotho aa 400-500. The exact sequence is proprietary.
    Database link: Q9UEF7

  • ポジティブ･コントロール

    • Human testis, fetal kidney and fetal thymus lysates; Human clear cell carcinoma kidney tissue, Human kidney tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab181373の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (1)
1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified, use 1/100 - 1/250.
WB
1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62, 116 kDa).
特記事項
IHC-P
1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified, use 1/100 - 1/250.
WB
1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62, 116 kDa).
追加情報
Is unsuitable for ICC/IF.

ターゲット情報

  • 機能

    May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1.
    The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.

  • 組織特異性

    Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney.

  • 関連疾患

    Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement.

  • 配列類似性

    Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.

  • ドメイン

    Contains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 239, and the second one lacks the essential Glu active site residue at position 872.

  • 翻訳後修飾

    N-glycosylated.

  • 細胞内局在

    Secreted and Cell membrane. Isoform 1 shedding leads to a soluble peptide.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Kl antibody
      • KLOT_HUMAN antibody
      • Klotho peptide antibody

    画像

    • Western blot - Anti-Klotho antibody [EPR6856] (ab181373)
      Western blot - Anti-Klotho antibody [EPR6856] (ab181373)
      All lanes : Anti-Klotho antibody [EPR6856] (ab181373) at 5 µg/ml

      Lane 1 : Wild Type (WT) Mouse Kidney Tissue Lysate
      Lane 2 : Knock Out (KO) Mouse Kidney Tissue Lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 62, 116 kDa



      The blots were produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane were blocked for an hour before being incubated with ab181373  (rabbit anti-Klotho antibody; diluted 5 ug/ml) for 24 hours at 4°C. Antibody binding was detected using infrared labelled goat anti-rabbit (green) antibody at 1:10,000 dilutions for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody [EPR6856] (ab181373)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody [EPR6856] (ab181373)

      Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Klotho with unpurified ab181373 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-Klotho antibody [EPR6856] (ab181373)
      Western blot - Anti-Klotho antibody [EPR6856] (ab181373)
      All lanes : Anti-Klotho antibody [EPR6856] (ab181373) at 1/1000 dilution (purified)

      Lane 1 : Human fetal kidney
      Lane 2 : Human testis
      Lane 3 : Human fetal thymus

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 62, 116 kDa
      Observed band size: 62 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody [EPR6856] (ab181373)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody [EPR6856] (ab181373)

      Immunohistochemical staining of paraffin embedded human kidney with purified ab181373 at a working dilution of 1 in 300. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • Western blot - Anti-Klotho antibody [EPR6856] (ab181373)
      Western blot - Anti-Klotho antibody [EPR6856] (ab181373)
      All lanes : Anti-Klotho antibody [EPR6856] (ab181373) at 1/1000 dilution (unpurified)

      Lane 1 : Human fetal kidney lysates
      Lane 2 : Human testis lysates
      Lane 3 : Human fetal thymus lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 62, 116 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody [EPR6856] (ab181373)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody [EPR6856] (ab181373)

      Immunohistochemical analysis of paraffin-embedded human kidney clear cell carcinoma tissue labeling Klotho with unpurified ab181373 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Klotho antibody [EPR6856] (ab181373)
      Anti-Klotho antibody [EPR6856] (ab181373)

    参考文献 (30)

    ab181373 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab181373 は 30 報の論文で使用されています。

    • Li S  et al. Abnormally decreased renal Klotho is linked to endoplasmic reticulum-associated degradation in mice. Int J Med Sci 19:321-330 (2022). PubMed: 35165517
    • Xiong G  et al. Long non-coding RNA MEG3 regulates the progress of osteoarthritis by regulating the miR-34a/Klotho axis. Ann Transl Med 10:454 (2022). PubMed: 35571440
    • Li C  et al. Comprehensive Profiling Reveals Distinct Microenvironment and Metabolism Characterization of Lung Adenocarcinoma. Front Genet 12:619821 (2021). PubMed: 34122500
    • Zhu L  et al. Klotho inhibits H2 O2 -induced oxidative stress and apoptosis in periodontal ligament stem cells by regulating UCP2 expression. Clin Exp Pharmacol Physiol 48:1412-1420 (2021). PubMed: 34174105
    • Li M  et al. METTL14 promotes glomerular endothelial cell injury and diabetic nephropathy via m6A modification of α-klotho. Mol Med 27:106 (2021). PubMed: 34503454
    View all Publications for this product

    レビューと Q&A

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    レビューを送る 質問を送る

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Klotho antibody [EPR6856]

     Poor
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrat pH6.0
    Permeabilization
    No
    Specification
    Kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    投稿 Apr 11 2022

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