Anti-KLF3 抗体 (ab154531)
Key features and details
- Rabbit polyclonal to KLF3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-KLF3 antibody -
製品の詳細
Rabbit polyclonal to KLF3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Pig -
免疫原
Recombinant fragment, corresponding to a region within amino acids 153-334 of Human KLF3 (UniProt P57682).
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ポジティブ・コントロール
- A549 and HCT116 cell lysates; SCC4 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154531の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ターゲット情報
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機能
Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis. -
配列類似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
翻訳後修飾
Sumoylated by SUMO1. Sumoylation is enhanced by PIAS1, PIAS2alpha and PIAS2beta, and PIAS4, but not by Pc2. Enhances transcriptional repression, but has no effect on DNA binding. Sumoylation on Lys-198 is the major site. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 530358 Cow
- Entrez Gene: 488836 Human
- Entrez Gene: 51274 Human
- Entrez Gene: 16599 Mouse
- Entrez Gene: 100174960 Pig
- Entrez Gene: 114845 Rat
- Omim: 609392 Human
- SwissProt: P57682 Human
see all -
別名
- Basic krueppel-like factor antibody
- Basic Kruppel like factor antibody
- BKLF3 antibody
see all
画像
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Anti-KLF3 antibody (ab154531) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 39 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF3 antibody (ab154531)Immunohistochemical analysis of paraffin-embedded SCC4 xenograft tissue labeling KLF3 with ab154531 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab154531 は 6 報の論文で使用されています。
- Wu C et al. Exosome miR-23a-3p from Osteoblast Alleviates Spinal Cord Ischemia/Reperfusion Injury by Down-Regulating KLF3-Activated CCNL2 Transcription. Dev Neurosci 44:121-130 (2022). PubMed: 34937039
- Zhang J et al. Long non-coding RNA NEAT1 regulates glioma cell proliferation and apoptosis by competitively binding to microRNA-324-5p and upregulating KCTD20 expression. Oncol Rep 46:N/A (2021). PubMed: 33982764
- Li J et al. Inhibition of the cell migration, invasion and chemoresistance of colorectal cancer cells through targeting KLF3 by miR-365a-3p. J Cancer 12:6155-6164 (2021). PubMed: 34539888
- Liu JQ et al. lncRNA KLF3-AS1 Suppresses Cell Migration and Invasion in ESCC by Impairing miR-185-5p-Targeted KLF3 Inhibition. Mol Ther Nucleic Acids 20:231-241 (2020). PubMed: 32193151
- Wu S et al. Circ_0084043 promotes cell proliferation and glycolysis but blocks cell apoptosis in melanoma via circ_0084043-miR-31-KLF3 axis. Open Life Sci 15:774-786 (2020). PubMed: 33817265
- Wang X et al. RNA sequencing analysis reveals protective role of kruppel-like factor 3 in colorectal cancer. Oncotarget 8:21984-21993 (2017). IHC-P ; Human . PubMed: 28423541