AB154531

Anti-KLF3 抗体

Anti-KLF3 antibody

JP Deleterious:医薬用外劇物

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(7 Publications)

Rabbit Polyclonal KLF3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KLF3 aa 150 to C-terminus.

別名を表示する

BKLF, KLF3, Krueppel-like factor 3, Basic krueppel-like factor, CACCC-box-binding protein BKLF, TEF-2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF3 antibody (AB154531)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF3 antibody (AB154531)

Immunohistochemical analysis of paraffin-embedded SCC4 xenograft tissue labeling KLF3 with ab154531 at 1/100 dilution.

Unknown

Western blot - Anti-KLF3 antibody (AB154531)

10% SDS PAGE

All lanes:

Western blot - Anti-KLF3 antibody (ab154531) at 1/1000 dilution

All lanes:

HCT116 whole cell lysate at 30 µg

Predicted band size: 39 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human KLF3 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P57682

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

KLF3 also known as BKLF (Basic Krüppel-like Factor) is a transcription factor weighing approximately 40 kDa. It regulates gene expression by binding to specific DNA sequences generally influencing various cellular processes. KLF3 is expressed in diverse tissues such as hematopoietic cells and adipose tissues among others. Its presence is observed in both normal physiological conditions and under certain stresses indicating a broader regulatory role.

Biological function summary

KLF3 acts as a major regulator of cell differentiation and proliferation. It functions as part of a transcriptional complex interacting with co-factors to modulate gene networks. Through its zinc finger domains KLF3 binds to DNA and influences the transcription of genes involved in erythropoiesis the process that produces red blood cells and adipogenesis the formation of fat cells. These biological roles suggest that KLF3 maintains homeostasis in tissue development and maturation.

Pathways

Multiple cellular events involve KLF3. It significantly contributes to the TGF-beta signaling pathway and the erythropoiesis pathway. Within these pathways KLF3 interacts with other proteins like TGF-beta receptors and other transcription factors like GATA1. This interaction helps drive transcriptional changes necessary for these pathways' completion indicating KLF3's integral role.

KLF3 plays a part in several conditions including obesity and anemia. In obesity alteration in KLF3 expression affects adipogenesis impacting fat cell formation and accumulation. In anemia KLF3 dysregulation leads to improper erythropoiesis due to its interaction with proteins like GATA1 and EPOR (erythropoietin receptor). These associations underline KLF3's potential implications in managing metabolic and blood disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis (By similarity).

See full target information KLF3

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:8038-8050 PubMed35311620

2022

Fat mass and obesity associated (FTO)-mediated N6-methyladenosine modification of Krüppel-like factor 3 (KLF3) promotes osteosarcoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Jian Shan,Wen-Xiang Gu,Gang Duan,Hong-Liang Chen

Developmental neuroscience 44:121-130 PubMed34937039

2021

Exosome miR-23a-3p from Osteoblast Alleviates Spinal Cord Ischemia/Reperfusion Injury by Down-Regulating KLF3-Activated CCNL2 Transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Wu,Qinghua Zhu,Yi Yao,Zhaoyang Shi,Chaojie Jin,Leilei Chen

Journal of Cancer 12:6155-6164 PubMed34539888

2021

Inhibition of the cell migration, invasion and chemoresistance of colorectal cancer cells through targeting KLF3 by miR-365a-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Rubing Mo,Linmei Zheng

Oncology reports 46: PubMed33982764

2021

Long non‑coding RNA NEAT1 regulates glioma cell proliferation and apoptosis by competitively binding to microRNA‑324‑5p and upregulating KCTD20 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jiale Zhang,Yangyang Li,Yuqi Liu,Guangzhi Xu,Yue Hei,Xiaoming Lu,Weiping Liu

Open life sciences 15:774-786 PubMed33817265

2020

Circ_0084043 promotes cell proliferation and glycolysis but blocks cell apoptosis in melanoma via circ_0084043-miR-31-KLF3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Songjiang Wu,Yuhan Tang,Wenli Liu

Molecular therapy. Nucleic acids 20:231-241 PubMed32193151

2020

lncRNA KLF3-AS1 Suppresses Cell Migration and Invasion in ESCC by Impairing miR-185-5p-Targeted KLF3 Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Qi Liu,Ming Deng,Nan-Nan Xue,Ting-Xuan Li,Yue-Xin Guo,Liang Gao,Di Zhao,Rui-Tai Fan

Oncotarget 8:21984-21993 PubMed28423541

2017

RNA sequencing analysis reveals protective role of kruppel-like factor 3 in colorectal cancer.

Applications

IHC-P

Species

Human

Xiaohong Wang,Zhonghua Jiang,Yu Zhang,Xiang Wang,Li Liu,Zhining Fan

View all publications

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Abcam product promise

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Anti-KLF3 抗体

Anti-KLF3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF3 antibody (AB154531)

Western blot - Anti-KLF3 antibody (AB154531)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (7)

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise