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AB314431

Anti-KLF2 抗体 [EPR23420-40] - BSA and Azide free

Anti-KLF2 antibody [EPR23420-40] - BSA and Azide free

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Rabbit Recombinant Monoclonal KLF2 antibody. Carrier free. Suitable for WB and reacts with Mouse samples.

別名を表示する

Lklf, Klf2, Krueppel-like factor 2, Lung krueppel-like factor

3 Images
Western blot - Anti-KLF2 antibody [EPR23420-40] - BSA and Azide free (AB314431)
  • WB

Supplier Data

Western blot - Anti-KLF2 antibody [EPR23420-40] - BSA and Azide free (AB314431)

This data was developed using ab314430, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. The identity of the bands above 38 kDa is unknown. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : Lane 1 : 37 seconds Lane 2 : 59 seconds

All lanes:

Western blot - Anti-KLF2 antibody [EPR23420-40] (ab314430) at 1/1000 dilution

Lane 1:

ES-D3 (mouse embryonic pluripotent stem Cell) whole cell lysate at 20 µg

Lane 2:

Mouse lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 38 kDa

false

Western blot - Anti-KLF2 antibody [EPR23420-40] - BSA and Azide free (AB314431)
  • WB

Supplier Data

Western blot - Anti-KLF2 antibody [EPR23420-40] - BSA and Azide free (AB314431)

This data was developed usingab314430, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-KLF2 antibody [EPR23420-40] (ab314430) at 1/1000 dilution

Lane 1:

F9 (mouse embryonal carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate 20 µg at 20 µg

Lane 2:

F9 transfected with siRNAs specifically targeting KLF2 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 38 kDa,37 kDa

false

Exposure time: 59s

Western blot - Anti-KLF2 antibody [EPR23420-40] - BSA and Azide free (AB314431)
  • WB

Supplier Data

Western blot - Anti-KLF2 antibody [EPR23420-40] - BSA and Azide free (AB314431)

This data was developed usingab314430, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : brain, retina, liver. The identity of the bands above 38 kDa and around 18 kDa are unknown. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-KLF2 antibody [EPR23420-40] (ab314430) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Mouse retina tissue lysate at 20 µg

Lane 5:

Mouse liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (<a href='/products/primary-antibodies/gapdh-antibody-epr16891-loading-control-ab181602'>ab181602</a>) at 1/200000 dilution

Observed band size: 38 kDa,37 kDa

false

Exposure time: 81s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR23420-40

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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製品の詳細

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: 100% PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

KLF2 also known as Kruppel-like factor 2 is a transcription factor with a molecular mass of approximately 37 kDa. It belongs to the Kruppel-like family of transcription factors which share zinc finger domains. KLF2 regulates gene expression by binding to specific DNA sequences in promoters influencing cellular processes. Researchers have localized its expression predominantly in the vascular endothelium the lungs and the spleen. In these tissues KLF2 influences endothelial cell function and vascular biology.
Biological function summary

KLF2 mediates cellular processes including endothelial cell maintenance and vascular development. As a transcription factor KLF2 does not operate as part of a multi-protein complex but rather performs its regulatory function individually by modulating target gene expression. KLF2 activates genes associated with anti-inflammatory and anti-thrombotic properties in endothelial cells contributing to vascular homeostasis. Its role is key in maintaining the balance between pro- and anti-inflammatory signals in the vascular system.

Pathways

KLF2 integrates its function into various signaling pathways notably the MEK/ERK and PI3K/Akt pathways. KLF2 modulates these pathways to control proliferation differentiation and apoptosis in endothelial cells. In particular KLF2-induced gene expression influences these pathways by interacting with other proteins such as MEK and ERK proteins that regulate cell survival and inflammatory responses. These interactions highlight the relevance of KLF2 in cellular signaling important for vascular health.

KLF2 has significant implications for cardiovascular diseases and atherosclerosis. Research associates KLF2 with protective roles against the development of atherosclerosis due to its influence on endothelial function and inflammation. When KLF2 expression is compromised the risk for thrombosis and vascular inflammation increases contributing to cardiovascular conditions. Additionally studies link KLF2 to the pathophysiology of diabetes where aberrant KLF2 expression disrupts normal endothelial functions exacerbating diabetic complications. KLF2 interacts with proteins like NF-kB in such conditions impacting the expression of genes involved in inflammation and metabolism.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Transcription factor that binds to the CACCC box in the promoter of target genes such as HBB/beta globin or NOV and activates their transcription. Might be involved in transcriptional regulation by modulating the binding of the RARA nuclear receptor to RARE DNA elements (By similarity).
See full target information Klf2
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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。
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関連製品

Select an associated product type
Alternative Version
Primary Antibodies

AB314430

Anti-KLF2 antibody [EPR23420-40]

primary-antibodies

klf2-antibody-epr23420-40-ab314430

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