Anti-KLF16 抗体 (ab187973)
Key features and details
- Rabbit polyclonal to KLF16
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-KLF16 antibody -
製品の詳細
Rabbit polyclonal to KLF16 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment corresponding to Human KLF16 aa 200 to the C-terminus.
Database link: Q9BXK1 -
ポジティブ･コントロール
- IHC-P: Human small intestine tissue; ICC: MCF7 whole cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187973の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
| 特記事項 |
|---|
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
ターゲット情報
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機能
Transcription factor that binds GC and GT boxes and displaces Sp1 and Sp3 from these sequences. Modulates dopaminergic transmission in the brain. -
配列類似性
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 83855 Human
- Entrez Gene: 118445 Mouse
- Entrez Gene: 690820 Rat
- Omim: 606139 Human
- SwissProt: Q9BXK1 Human
- SwissProt: P58334 Mouse
- Unigene: 136280 Human
- Unigene: 41513 Mouse
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別名
- AI843742 antibody
- Basic transcription element binding protein 4 antibody
- Basic transcription element-binding protein 4 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF16 antibody (ab187973)
Immunohistochemical analysis of paraffin-embedded human small intestine tissue labeling KLF16 in the nucleus of glandular cells with ab187973 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-KLF16 antibody (ab187973)Immunocytochemical analysis of MCF7 (Human breast adenocarcinoma cell line) whole cells labeling KLF16 in the nucleus with ab187973 at 2 µg/ml.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab187973 は 2 報の論文で使用されています。
- Yang Z et al. SF3B4 promotes Twist1 expression and clear cell renal cell carcinoma progression by facilitating the export of KLF 16 mRNA from the nucleus to the cytoplasm. Cell Death Dis 14:26 (2023). PubMed: 36639679
- Jiao X et al. Kruppel like factor 16 promotes lung adenocarcinoma progression by upregulating lamin B2. Bioengineered 13:9482-9494 (2022). PubMed: 35387557