Anti-KLC1 抗体 (ab187179)
Key features and details
- Rabbit polyclonal to KLC1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-KLC1 antibody
KLC1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KLC1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant full length protein corresponding to Human KLC1.
Database link: Q07866 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187179の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
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| WB |
1/500 - 1/2000. Predicted molecular weight: 65 kDa.
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| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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WB
1/500 - 1/2000. Predicted molecular weight: 65 kDa. |
ターゲット情報
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機能
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity. -
組織特異性
Found in a variety of tissues. Mostly abundant in brain and spine. -
配列類似性
Belongs to the kinesin light chain family.
Contains 6 TPR repeats. -
翻訳後修飾
Isoform I is phosphorylated on Ser-600. Isoform J is phosphorylated on Ser-631. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 3831 Human
- Entrez Gene: 16593 Mouse
- Omim: 600025 Human
- SwissProt: Q07866 Human
- SwissProt: O88447 Mouse
- Unigene: 20107 Human
- Unigene: 657678 Human
- Unigene: 278357 Mouse
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別名
- Kinesin 2 60/70kDa antibody
- Kinesin light chain 1 antibody
- Kinesin2 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody (ab187179)Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab187179. Blue DAPI for nuclear staining.
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Western blot - Anti-KLC1 antibody (ab187179)All lanes : Anti-KLC1 antibody (ab187179) at 1/500 dilution
Lane 1 : SW480 cell extract
Lane 2 : 22RV1 cell extract
Lane 3 : BT474 cell extract
Lane 4 : MCF7 cell extract
Lane 5 : Mouse brain cell extract
Predicted band size: 65 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab187179 は 4 報の論文で使用されています。
- Komori T et al. A CRISPR-del-based pipeline for complete gene knockout in human diploid cells. J Cell Sci 136:N/A (2023). PubMed: 36762651
- Dinamarca MC et al. Complex formation of APP with GABAB receptors links axonal trafficking to amyloidogenic processing. Nat Commun 10:1331 (2019). PubMed: 30902970
- Holt I et al. Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle. Sci Rep 9:14202 (2019). PubMed: 31578382
- Jang WH et al. Interaction of FUN14 domain containing 1, a mitochondrial outer membrane protein, with kinesin light chain 1 via the tetratricopeptide repeat domain. Biomed Rep 6:46-50 (2017). PubMed: 28123706