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リコンビナントRabMAb

Anti-Kir6.2/BIR 抗体 [EPR25229-115] (BSA and Azide free) (ab291089)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] - BSA and Azide free (ab291089)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] - BSA and Azide free (ab291089)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] - BSA and Azide free (ab291089)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] - BSA and Azide free (ab291089)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] - BSA and Azide free (ab291089)
  • Anti-Kir6.2/BIR antibody [EPR25229-115] - BSA and Azide free (ab291089)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25229-115] to Kir6.2/BIR - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Kir6.2/BIR antibody [EPR25229-115] (BSA and Azide free)
    Kir6.2/BIR 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25229-115] to Kir6.2/BIR - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-Pmore details
    適用なし: Flow Cyt (Intra),ICC/IF,IP or WB

  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Mouse and rat: cardiac muscle and pancreas. HEK-293T transfected with a mouse KCNJ11 expression vector containing a his tag. HEK-293T transfected with rat KCNJ11 expression vector containing a his tag.

  • 特記事項

    ab291089 is a carrier free version of ab291074. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

関連製品

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab291089の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF,IP or WB.

ターゲット情報

  • 関連性

    ATP-sensitive potassium (K(ATP)) channels are found in endocrine cells, neurons and both smooth and striated muscle, where they play an important role in controlling insulin secretion and vascular tone, and protect neurons under metabolic stress. Kir6.2 is a member of the inward rectifier potassium channel family, which is characterised by a greater tendency to allow potassium flow into the cell rather than out of it. It associates with the sulphonylurea receptor SUR1/ABCC8 to form a subfamily of K(ATP) channels that, when mutated or misregulated, are associated with forms of hyperinsulinemic hypoglycemia, neonatal diabetes, or pre-disposition to type 2 diabetes mellitus.

  • 細胞内局在

    Cell Membrane; Multi-pass membrane protein

  • 参照データベース

    • 別名

      • ATP sensitive inward rectifier potassium channel 11 antibody
      • Beta cell inward rectifier subunit antibody
      • BIR antibody
      • HHF 2 antibody
      • HHF2 antibody
      • IKATP antibody
      • Inward rectifier K(+) channel Kir6.2 antibody
      • Inwardly rectifying potassium channel KIR6.2 antibody
      • IRK 11 antibody
      • IRK11 antibody
      • KCNJ11 antibody
      • Kir 6.2 antibody
      • Kir6.2 antibody
      • MGC133230 antibody
      • PHHI antibody
      • Potassium channel inwardly rectifing subfamily J member 11 antibody
      • Potassium channel, inwardly rectifying subfamily J member 11 antibody
      • Potassium inwardly rectifying channel J11 antibody
      • TNDM 3 antibody
      • TNDM3 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] - BSA and Azide free (ab291089)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] - BSA and Azide free (ab291089)

      This data was developed using ab291074, the same antibody clone in a different buffer formulation. 

      Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle labeling Kir6.2/BIR with ab291074 at 1/2000 dilution, followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the intercalated disc in mouse cardiac muscle.
      The section was incubated with ab291074 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

      Secondary antibody control: PBS was used instead of primary antibody followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] - BSA and Azide free (ab291089)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] - BSA and Azide free (ab291089)

      This data was developed using ab291074, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse pancreas labeling Kir6.2/BIR with ab291074 at 1/2000 dilution, followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the islet in mouse pancreas (PMID: 24101510). The section was incubated with ab291074 for 30 mins at room temperature.

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

      Secondary antibody control: PBS was used instead of primary antibody followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] - BSA and Azide free (ab291089)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] - BSA and Azide free (ab291089)

      This data was developed using ab291074, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat cardiac muscle labeling Kir6.2/BIR with ab291074 at 1/2000 dilution, followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the intercalated disc in rat cardiac muscle (PMID 23066018). The section was incubated with ab291074 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

      Secondary antibody control: PBS was used instead of primary antibody followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] - BSA and Azide free (ab291089)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] - BSA and Azide free (ab291089)

      This data was developed using ab291074, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat pancreas labeling Kir6.2/BIR with ab291074 at 1/2000 dilution, followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the islet in rat pancreas. The section was incubated with ab291074 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

      Secondary antibody control: PBS was used instead of primary antibody followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] - BSA and Azide free (ab291089)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] - BSA and Azide free (ab291089)

      This data was developed using ab291074, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded (A) HEK-293T transfected with a mouse KCNJ11 expression vector containing a his tag. (B) HEK-293T transfected with rat KCNJ11 expression vector containing a his tag. (C) HEK-293T cells transfected with empty vector containing a his tag labeling Kir6.2/BIR with ab291074 at 1/2000 dilution, followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with a mouse KCNJ11 expression vector containing a his tag; positive staining on (B) HEK-293T transfected with rat KCNJ11 expression vector containing a his tag. No staining on (C) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab291074 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab291074 for 30 mins at room temperature.
      Counterstained with Hematoxylin. 

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

      Secondary antibody control: PBS was used instead of primary antibody followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    • Anti-Kir6.2/BIR antibody [EPR25229-115] - BSA and Azide free (ab291089)
      Anti-Kir6.2/BIR antibody [EPR25229-115] - BSA and Azide free (ab291089)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab291089 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab291089 は論文での使用が確認できていません。

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