Anti-Kir6.2/BIR 抗体 [EPR25229-115] (ab291074)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle labeling Kir6.2/BIR with ab291074 at 1/2000 dilution, followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the intercalated disc in mouse cardiac muscle.
The section was incubated with ab291074 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. 

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

Secondary antibody control: PBS was used instead of primary antibody followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). 

Immunohistochemical analysis of paraffin-embedded mouse pancreas labeling Kir6.2/BIR with ab291074 at 1/2000 dilution, followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the islet in mouse pancreas (PMID: 24101510). The section was incubated with ab291074 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

Secondary antibody control: PBS was used instead of primary antibody followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Immunohistochemical analysis of paraffin-embedded rat cardiac muscle labeling Kir6.2/BIR with ab291074 at 1/2000 dilution, followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the intercalated disc in rat cardiac muscle (PMID 23066018). The section was incubated with ab291074 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. 

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

Secondary antibody control: PBS was used instead of primary antibody followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Immunohistochemical analysis of paraffin-embedded rat pancreas labeling Kir6.2/BIR with ab291074 at 1/2000 dilution, followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the islet in rat pancreas. The section was incubated with ab291074 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. 

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

Secondary antibody control: PBS was used instead of primary antibody followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T transfected with a mouse KCNJ11 expression vector containing a his tag. (B) HEK-293T transfected with rat KCNJ11 expression vector containing a his tag. (C) HEK-293T cells transfected with empty vector containing a his tag labeling Kir6.2/BIR with ab291074 at 1/2000 dilution, followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with a mouse KCNJ11 expression vector containing a his tag; positive staining on (B) HEK-293T transfected with rat KCNJ11 expression vector containing a his tag. No staining on (C) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab291074 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. The section was incubated with ab291074 for 30 mins at room temperature.
Counterstained with Hematoxylin. 

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

Secondary antibody control: PBS was used instead of primary antibody followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).