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  1. Link

    products/primary-antibodies/kir21kcnj2-antibody-ab65796.pdf

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Neuroscience Neurotransmission Receptors / Channels Potassium Channels
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Anti-Kir2.1/KCNJ2 抗体 (ab65796)

  • Datasheet
Reviews (2)Q&A (3)References (14)

Product price, shipping and contact information

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Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunocytochemistry/ Immunofluorescence - Anti-Kir2.1/KCNJ2 antibody (ab65796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir2.1/KCNJ2 antibody (ab65796)

Key features and details

  • Rabbit polyclonal to Kir2.1/KCNJ2
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Kir2.1/KCNJ2 antibody [EPR4530] (ab109750)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Kir2.1/KCNJ2 antibody
    Kir2.1/KCNJ2 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Kir2.1/KCNJ2
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IHC-P, ICC/IFmore details
  • 種交差性

    交差種: Rat, Human
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    Constituent: Whole serum
  • Concentration information loading...
  • 精製度

    Whole antiserum
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Potassium Channels

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab65796の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/1000.
ICC/IF
1/1000.
特記事項
IHC-P
1/1000.
ICC/IF
1/1000.

ターゲット情報

  • 機能

    Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium or cesium.
  • 組織特異性

    Heart, brain, placenta, lung, skeletal muscle, and kidney. Diffusely distributed throughout the brain.
  • 関連疾患

    Defects in KCNJ2 are the cause of long QT syndrome type 7 (LQT7) [MIM:170390]; also called Andersen syndrome or Andersen cardiodysrhythmic periodic paralysis. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. LQT7 manifests itself as a clinical triad consisting of potassium-sensitive periodic paralysis, ventricular ectopy and dysmorphic features.
    Defects in KCNJ2 are the cause of short QT syndrome type 3 (SQT3) [MIM:609622]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death. SQT3 has a unique ECG phenotype characterized by asymmetrical T waves.
  • 配列類似性

    Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ2 subfamily.
  • 細胞内局在

    Membrane.
  • Target information above from: UniProt accession P63252 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 3759 Human
    • Entrez Gene: 29712 Rat
    • Omim: 600681 Human
    • SwissProt: P63252 Human
    • SwissProt: Q64273 Rat
    • Unigene: 1547 Human
    • Unigene: 44415 Rat
    • 別名

      • Cardiac inward rectifier potassium channel antibody
      • HHBIRK 1 antibody
      • HHBIRK1 antibody
      • HHIRK 1 antibody
      • HHIRK1 antibody
      • HIRK 1 antibody
      • hIRK1 antibody
      • Inward rectifier K antibody
      • Inward rectifier K(+) channel Kir2.1 antibody
      • Inward rectifier potassium channel 2 antibody
      • inwardly rectifying subfamily J member 2 antibody
      • IRK 1 antibody
      • IRK-1 antibody
      • IRK1 antibody
      • IRK2_HUMAN antibody
      • KCNJ2 antibody
      • KIR2.1 antibody
      • LQT 7 antibody
      • LQT7 antibody
      • Potassium channel antibody
      • Potassium channel inwardly rectifying subfamily J member 2 antibody
      • Potassium inwardly rectifying channel J2 antibody
      • Potassium inwardly rectifying channel subfamily J member 2 antibody
      • SQT 3 antibody
      • SQT3 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-Kir2.1/KCNJ2 antibody (ab65796)
      Immunocytochemistry/ Immunofluorescence - Anti-Kir2.1/KCNJ2 antibody (ab65796)
      ICC/IF image of ab65796 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65796, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir2.1/KCNJ2 antibody (ab65796)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir2.1/KCNJ2 antibody (ab65796)

      ab65796 staining Kir2.1/KCNJ2 in human colon.
      Left panel: with primary antibody at 1:1000 dilution. Right panel: isotype control.
      Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet download

      Download

    参考文献 (14)

    ab65796 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab65796 は 14 報の論文で使用されています。

    • Lu A  et al. Inhibition of Wnt/β-catenin signaling upregulates Nav 1.5 channels in Brugada syndrome iPSC-derived cardiomyocytes. Physiol Rep 11:e15696 (2023). PubMed: 37226398
    • Han JL  et al. CRISPRi gene modulation and all-optical electrophysiology in post-differentiated human iPSC-cardiomyocytes. Commun Biol 6:1236 (2023). PubMed: 38062109
    • Li W  et al. Protein and mRNA Quantification in Small Samples of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes in 96-Well Microplates. Methods Mol Biol 2485:15-37 (2022). PubMed: 35618896
    • Hansson O Biomarkers for neurodegenerative diseases. Nat Med 27:954-963 (2021). PubMed: 34083813
    • Akyuz E  et al. Unraveling the Role of Inwardly Rectifying Potassium Channels in the Hippocampus of an Aß(1-42)-Infused Rat Model of Alzheimer's Disease. Biomedicines 8:N/A (2020). PubMed: 32183098
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry free floating abreview for Anti-Kir2.1/BIK antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Brain striatum)
    Specification
    Brain striatum
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 May 12 2020

    Western blot abreview for Anti-Kir2.1/BIK antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Striatum tissues)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    20 µg
    Specification
    Striatum tissues
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 May 12 2020

    Question

    Many thanks for your reply. Would it be possible for you to send me some samples of these Abs to test? There are no pictures of westerns on your website for ab65796 and ab80969. I can send you pics of any westerns we do with them.

    For the western with ab109750 on your website, were the 293T cells transfected with Kir2.1? I don't think 293T cells express Kir2.1 endogenously.

    Also, if you could possibly find out whether HeLa cells need to be treated with staurosporine to get a good Kir2.1 signal, that would be very useful info.

    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Mar 02 2012

    Answer

    Thank you for your message.

    I am sorry to confirm that becuase we have over 70 000 products, it is not feasable for us to keep small sample vials to send to customers.

    I would like to reassure you that ab65796 and ab80969 have been tested in western blot, and we provide our 6 month guarantee for this application. However, as they have been tested externally, we do not currently have a WB image for them.

    Therefore, I can offer a discount off a future purchase if you buy either ab65796 or ab80969 now, use it WB and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody.

    It doesn’t matter whether the result is positive or negative, we would just really like to receive your feedback. Of course, if the results are negative you will be covered by our Abpromise guarantee and eligible for a refund or replacement.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed with this offer, and which of these two antibody you woudl like to try. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

    2. Purchase ab65796 or ab80969 either by phone, fax, or online (www.abcam.com).

    3. Use it in WB.

    4. Send us an image of the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated!

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    With regards to ab109750, I can confirm that 293T cells express Kir2.1 endogenously, and no treatment is needed. Other suppliers also used 293T as a positive control with Kir2.1 antibodies.

    The following paper describes Kir2.1 endogenously expressed in 293T cells:
    http://jp.physoc.org/content/563/3/725.full.pdf

    I hope this will be helpful to you. If you have any further questions, or if you would like to accept the testing offer, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Mar 02 2012

    Question

    Hi,

    I'm interested in seeing whether your anti-kir2.1 antibodies will cross-react with the zebrafish kir2.1 protein. However, for the 6 anti-kir2.1 antibodies that you stock there are no details regarding the region the antibodies are targeting, and therefore I can't be sure which antibody is the best suited for my needs. I've included the amino acid sequence of the zebrafish kir2.1 in this email in hopes that you would be able to tell me which antibody target shares the highest homology with it and therefore may be able to cross-react with the zebrafish protein.

    zebrafish kir2.1
    MGSVRANRYSIVSSEEDGMKLATAAVPNGYGNGKSKVHTRHQTQSRFVKKDGHCNVQFIN
    VSEKSQRYLADIFTTCVDIRWRWMFVIFCLAFLLSWLFFGCIFWLVAIFHGDLENNGHKC
    VSNVSSFTAAFLFSIETQTTIGYGYRYVTDECPIAVFMVVFQSIVGCIIDAFIIGAVMAK
    MAKPKKRNETLVFSHNATVAMRDNKLCLMWRVGNLRKSHLVEAHVRAQLLRSRTTAEREF
    IPLDQMDIDVGFDSGIDRIFLVSPITIVHEIDEDSPFYDMSKQEMENSDFEIVVILEGMV
    EATAMTTQCRSSYVANEILWAHRFEPVLFEEKNYYKVDYSRFHKTYEVPSTPLCSARDLA
    EKKYIISNSNSFCYENEVALSNKEEKEEGHGDSLGPGGTNTETSSDSEHSQATVPLEPRP
    LRRESEI
    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on May 17 2012

    Answer

    Thank you for contacting us.
    The immunogen of ab80969 shares 85% homology with Zebrafish so it is more likely to cross-react. Once you decide to buy the antibody I would like to inform you about the Abreview 100% discount programme. Please click the link
    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount
    We are also giving away free protein ladder with primary orders. Please check
    https://www.abcam.com/index.html?pageconfig=resource&rid=14968
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on May 17 2012

    Question

    Hi Technical,



    We have a customer who is interested in the following antibodies;



    ab85492, ab109750, ab65796, ab80969, ab45390, ab71574.



    Their question is as follows;



    I'm interested in seeing whether your anti-kir2.1 antibodies will cross-react with the zebrafish kir2.1 protein. However, for the 6 anti-kir2.1 antibodies that you stock there are no details regarding the region the antibodies are targeting, and therefore I can't be sure which antibody is the best suited for my needs. I've included the amino acid sequence of the zebrafish kir2.1 in this email in hopes that you would be able to tell me which antibody target shares the highest homology with it and therefore may be able to cross-react with the zebrafish protein.



    zebrafish kir2.1



    MGSVRANRYSIVSSEEDGMKLATAAVPNGYGNGKSKVHTRHQTQSRFVKKDGHCNVQFIN

    VSEKSQRYLADIFTTCVDIRWRWMFVIFCLAFLLSWLFFGCIFWLVAIFHGDLENNGHKC

    VSNVSSFTAAFLFSIETQTTIGYGYRYVTDECPIAVFMVVFQSIVGCIIDAFIIGAVMAK

    MAKPKKRNETLVFSHNATVAMRDNKLCLMWRVGNLRKSHLVEAHVRAQLLRSRTTAEREF

    IPLDQMDIDVGFDSGIDRIFLVSPITIVHEIDEDSPFYDMSKQEMENSDFEIVVILEGMV

    EATAMTTQCRSSYVANEILWAHRFEPVLFEEKNYYKVDYSRFHKTYEVPSTPLCSARDLA

    EKKYIISNSNSFCYENEVALSNKEEKEEGHGDSLGPGGTNTETSSDSEHSQATVPLEPRP

    LRRESEI



    According to the datasheet, they have not been tested with Zebrafish, so I would assume that you can’t confirm for them, but it is best to check. I will also mention to them of Abcam’s testing discount.



    Looking forward to your response.





    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on May 16 2012

    Answer

    Thank youand your customer very much for your interest in our antibodies.

    I checked all immunogens and homology to zebrafish (sequnec that your customer provided)of the Kir2.1 antibodies we offer at the moment.

    Only one antibody shows a sufficiently high homology to expect cross-reactivity: ab71574



    Therefore, I can offer a discount off a future purchase if your customerbuys ab71574 now, tests it inzebrafish and submits feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your customer'sfeedback. The discount would be to the value of ab71574 and can be used to order any product.

    If your customer is interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that your customer ( please include theire-mail address)would like to proceed and test ab71574 in zebrafish. I will then send a discount code. This code must be issued before purchasing ab71574 so please wait for my reply before ordering.

    2. Purchase ab71574 either by phone, fax, or online (www.abcam.com).

    3. Test it in zebrafish.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your customer's new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyproduct ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab71574turns out to be unsuitable for zebrafish, your customerwill still receive the discount ontheir next purchase after your Abreview has been submitted.

    Please let me know if you or your customer have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on May 16 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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