Anti-Kinetochore 抗体 (ab85996)
Key features and details
- Rabbit polyclonal to Kinetochore
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Kinetochore antibody
Kinetochore 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Kinetochore -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Xenopus tropicalis, Platypus
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免疫原
Synthetic peptide corresponding to Human Kinetochore aa 350-450.
Database link: P50748 -
ポジティブ･コントロール
- HeLa whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab85996 was affinity purified using an epitope specific to Kinetochore immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85996の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 250 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 250 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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関連性
The kinetochore is the protein structure in eukaryotes which assembles on the centromere and links the chromosome to microtubule polymers from the mitotic spindle during mitosis and meiosis. The kinetochore contains two regions: an inner kinetochore, which is tightly associated with the centromere DNA; and an outer kinetochore, which interacts with microtubules. Even the simplest kinetochores consist of more than 45 different proteins, many of which are conserved throughout eukaryote species. -
細胞内局在
Cytoplasmic and Nuclear -
参照データベース
- Entrez Gene: 9735 Human
- Omim: 607363 Human
- SwissProt: P50748 Human
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別名
- HsROD antibody
- Kinetochore associated 1 antibody
- KNTC1 antibody
see all
画像
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Western blot - Anti-Kinetochore antibody (ab85996)All lanes : Anti-Kinetochore antibody (ab85996) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 250 kDa
Observed band size: 250 kDa
Additional bands at: 115 kDa, 175 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds -
Immunoprecipitation - Anti-Kinetochore antibody (ab85996)Detection of Kinetochore by Western Blot of Immunprecipitate.
ab85996, at 1 µg/ml, staining Kinetochore in HeLa whole cell lysate immunoprecipitated using ab8599 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab85996 は 1 報の論文で使用されています。
- Liu CT et al. shRNA-mediated knockdown of KNTC1 suppresses cell viability and induces apoptosis in esophageal squamous cell carcinoma. Int J Oncol 54:1053-1060 (2019). PubMed: 30628654