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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Kinesin Heavy Chain 2 抗体 [EPR10740(B)] (ab154868)

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Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
  • Immunocytochemistry/ Immunofluorescence - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
  • Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
  • Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10740(B)] to Kinesin Heavy Chain 2
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)]
    Kinesin Heavy Chain 2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10740(B)] to Kinesin Heavy Chain 2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human fetal brain tissue lysate; Jurkat, 293T cell lysate; Human thyroid carcinoma tissue; Jurkat cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR10740(B)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab154868の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 187 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 187 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 関連性

    Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure which includes a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. An expansive phylogenetic tree of kinesins and kinesin-related proteins (KRPs) has been assembled based on this information.

  • 細胞内局在

    Cytoplasm, cytoskeleton

  • 参照データベース

    • 別名

      • HK2 antibody
      • KIF2 antibody
      • Kinesin heavy chain member 2 antibody
      • KNS2 antibody

    画像

    • Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
      Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Kinesin Heavy Chain 2 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab154868 observed at 100 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab154868 was shown to recognize Kinesin Heavy Chain 2 in wild-type HAP1 cells as signal was lost at the expected MW in Kinesin Heavy Chain 2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Kinesin Heavy Chain 2 knockout samples were subjected to SDS-PAGE. Ab154868 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
      Immunocytochemistry/ Immunofluorescence - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
      Immunofluorescent analysis of Jurkat cells labeling Kinesin Heavy Chain 2 with ab154868 at 1/100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)

      Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma labeling Kinesin Heavy Chain 2 with ab154868 at 1/50.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
      Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
      All lanes : Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868) at 1/1000 dilution

      Lane 1 : Human fetal brain tissue lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 187 kDa

    • Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
      Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)

    参考文献 (0)

    ab154868 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab154868 は論文での使用が確認できていません。

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