Anti-KIF5A + KIF5B + KIF5C 抗体 (ab62104)
Key features and details
- Rabbit polyclonal to KIF5A + KIF5B + KIF5C
- Suitable for: IHC-P, ICC/IF, IHC-Fr, IP, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-KIF5A + KIF5B + KIF5C antibody
KIF5A + KIF5B + KIF5C 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KIF5A + KIF5B + KIF5C -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, IHC-Fr, IP, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human KIF5A + KIF5B + KIF5C (C terminal).
Database link: Q12840 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62104の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
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| ICC/IF | (1) |
1/500.
|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
|
| IP | (1) |
Use at an assay dependent concentration.
See Abreview. |
| WB | (1) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 116 kDa (predicted molecular weight: 110 kDa).
|
| 特記事項 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
ICC/IF
1/500. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. See Abreview. |
|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 116 kDa (predicted molecular weight: 110 kDa). |
ターゲット情報
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関連性
The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. KIF5 has at least three known isoforms; this antibody recognizes all three. Mutations in one of the isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias. -
細胞内局在
Uniformly distributed between soma and neurites in hippocampal neurons -
参照データベース
- Entrez Gene: 3798 Human
- Entrez Gene: 3799 Human
- Entrez Gene: 3800 Human
- SwissProt: O60282 Human
- SwissProt: P33176 Human
- SwissProt: Q12840 Human
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別名
- KIF5A antibody
- KIF5B antibody
- KIF5C antibody
see all
画像
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Western blot - Anti-KIF5A + KIF5B + KIF5C antibody (ab62104)Lane 1 : Anti-KIF5A + KIF5B + KIF5C antibody (ab62104) at 0.5 µg/ml
Lane 2 : Anti-KIF5A + KIF5B + KIF5C antibody (ab62104) at 1 µg/ml
All lanes : K562 cell lysate
Predicted band size: 110 kDa
Observed band size: 116 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-KIF5A + KIF5B + KIF5C antibody (ab62104)ab62104 at 5 µg/ml staining KIF5A + KIF5B + KIF5C in human K562 cells by Immunocytochemistry.
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Immunocytochemistry/ Immunofluorescence - Anti-KIF5A + KIF5B + KIF5C antibody (ab62104)Immunofluorescence of KIF5 in K562 cells using ab62104 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab62104 は 5 報の論文で使用されています。
- Wakatsuki S et al. Small noncoding vault RNA modulates synapse formation by amplifying MAPK signaling. J Cell Biol 220:N/A (2021). PubMed: 33439240
- Thillaiappan NB et al. Ca2+signals initiate at immobile IP3receptors adjacent to ER-plasma membrane junctions. Nat Commun 8:1505 (2017). PubMed: 29138405
- Farías GG et al. BORC/kinesin-1 ensemble drives polarized transport of lysosomes into the axon. Proc Natl Acad Sci U S A 114:E2955-E2964 (2017). PubMed: 28320970
- Sherman MA et al. Soluble Conformers of Aß and Tau Alter Selective Proteins Governing Axonal Transport. J Neurosci 36:9647-58 (2016). PubMed: 27629715
- Su B et al. Brain-derived neurotrophic factor (BDNF)-induced mitochondrial motility arrest and presynaptic docking contribute to BDNF-enhanced synaptic transmission. J Biol Chem 289:1213-26 (2014). Rat . PubMed: 24302729