Anti-KIF4A/KIF4 抗体 (ab3815)
Key features and details
- Goat polyclonal to KIF4A/KIF4
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-KIF4A/KIF4 antibody
KIF4A/KIF4 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to KIF4A/KIF4 -
由来種
Goat -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Pig -
免疫原
Synthetic peptide corresponding to Human KIF4A/KIF4 aa 1219-1232 (C terminal).
Sequence:SFFSGCSPIEEEAH
(Peptide available asab22955) -
ポジティブ・コントロール
- WB: NIH/3T3 nuclear lysate. ICC/IF: U-2 OS cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3815の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (2) |
Use a concentration of 10 µg/ml.
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WB | (1) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 140 kDa.
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特記事項 |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 140 kDa. |
ターゲット情報
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機能
Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization. -
組織特異性
Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung. -
配列類似性
Belongs to the kinesin-like protein family. Chromokinesin subfamily.
Contains 1 kinesin-motor domain. -
細胞内局在
Nucleus matrix. Cytoplasm > cytoskeleton > spindle. Midbody. Chromosome. Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis. - Information by UniProt
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参照データベース
- Entrez Gene: 24137 Human
- Entrez Gene: 16571 Mouse
- Entrez Gene: 84393 Rat
- NCBI: 7305205 Human
- Omim: 300521 Human
- SwissProt: O95239 Human
- SwissProt: P33174 Mouse
- Unigene: 648326 Human
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別名
- Chromokinesin antibody
- Chromokinesin-A antibody
- Chromosome associated kinesin KIF4A antibody
see all
画像
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All lanes : Anti-KIF4A/KIF4 antibody (ab3815) at 2 µg/ml
Lane 1 : NIH/3T3 nuclear lysate
Lane 2 : Human ovary lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 140 kDa -
Immunocytochemical analysis of paraformaldehyde-fixed U-2 OS cells permeabilized with 0.15% Triton. Cells were incubated with ab3815 at 10 ug/ml for 1 hour followed by an Alexa Fluor® 488 secondary at 4 ug/ml (green). The nuclear stain is DAPI (blue). Negative control is unimmunized goat IgG at 10 ug/ml, followed by an Alexa Fluor® 488 secondary at 4 ug/ml (green).
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (6)
ab3815 は 6 報の論文で使用されています。
- Champion L et al. Dissociation of membrane-chromatin contacts is required for proper chromosome segregation in mitosis. Mol Biol Cell 30:427-440 (2019). PubMed: 30586323
- Lopez-Camacho C et al. Core binding factor ß (CBFß) is retained in the midbody during cytokinesis. J Cell Physiol 229:1466-74 (2014). PubMed: 24648201
- Hu CK et al. Midbody assembly and its regulation during cytokinesis. Mol Biol Cell 23:1024-34 (2012). ICC/IF . PubMed: 22278743
- Hu CK et al. Plk1 negatively regulates PRC1 to prevent premature midzone formation before cytokinesis. Mol Biol Cell 23:2702-11 (2012). ICC/IF ; Human . PubMed: 22621898
- Hu CK et al. KIF4 regulates midzone length during cytokinesis. Curr Biol 21:815-24 (2011). PubMed: 21565503
- Wilmeth LJ et al. Mutual dependence of Mob1 and the chromosomal passenger complex for localization during mitosis. Mol Biol Cell 21:380-92 (2010). Human . PubMed: 19955215