Anti-KIF20A 抗体 (ab70791)
Key features and details
- Rabbit polyclonal to KIF20A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-KIF20A antibody
KIF20A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KIF20A -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residue 840 and the C-terminus (residue 890) of human KIF20A. (NP_005724.1)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70791の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility. -
配列類似性
Belongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain. -
翻訳後修飾
Phosphorylated by PLK1 at Ser-528 during mitosis, creating a docking site for PLK1 and recruiting PLK1 at central spindle. -
細胞内局在
Golgi apparatus. Cytoplasm > cytoskeleton > spindle. - Information by UniProt
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参照データベース
- Entrez Gene: 527854 Cow
- Entrez Gene: 10112 Human
- Omim: 605664 Human
- SwissProt: Q29RT6 Cow
- SwissProt: O95235 Human
- Unigene: 718626 Human
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別名
- AA415432 antibody
- FLJ21151 antibody
- GG10 2 antibody
see all
画像
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Western blot - Anti-KIF20A antibody (ab70791)All lanes : Anti-KIF20A antibody (ab70791) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 100 kDa
Observed band size: 100 kDa
Additional bands at: 120 kDa, 31 kDa, 60 kDa, 68 kDa, 73 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunoprecipitation - Anti-KIF20A antibody (ab70791)HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab70791 at 1 µg/ml for WB, subsequent to using for IP at 3 µg/mg lysate.
Lane 2: IgG control.
Detection: Chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab70791 は 6 報の論文で使用されています。
- Luo K et al. Integrated bioinformatics analysis and experimental validation reveals hub genes of rheumatoid arthritis. Exp Ther Med 26:480 (2023). PubMed: 37745040
- Nishino M et al. Histone methyltransferase SUV39H1 regulates the Golgi complex via the nuclear envelope-spanning LINC complex. PLoS One 18:e0283490 (2023). PubMed: 37437070
- Yang Y et al. Development and Validation of a Combined Glycolysis and Immune Prognostic Model for Melanoma. Front Immunol 12:711145 (2021). PubMed: 34659201
- Hieda M et al. The SUN2-nesprin-2 LINC complex and KIF20A function in the Golgi dispersal. Sci Rep 11:5358 (2021). PubMed: 33686165
- Peng R et al. Identification of Core Genes Involved in the Metastasis of Clear Cell Renal Cell Carcinoma. Cancer Manag Res 12:13437-13449 (2020). PubMed: 33408516
- Bobustuc GC et al. MGMT inhibition in ER positive breast cancer leads to CDC2, TOP2A, AURKB, CDC20, KIF20A, Cyclin A2, Cyclin B2, Cyclin D1, ERa and Survivin inhibition and enhances response to temozolomide. Oncotarget 9:29727-29742 (2018). PubMed: 30038716