Anti-KIF14 抗体 (ab71155)
Key features and details
- Rabbit polyclonal to KIF14
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-KIF14 antibody
KIF14 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KIF14 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla
-
免疫原
Synthetic peptide corresponding to a region between residue 1600 and the C-terminus (residue 1648) of human KIF14 (NP_055690.1).
-
ポジティブ･コントロール
- Whole cell lysates from Hela cells and 293T cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab71155の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 210 kDa (predicted molecular weight: 186 kDa).
|
|
| IP |
Use at 2-5 µg/mg of lysate.
|
|
| IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Detects a band of approximately 210 kDa (predicted molecular weight: 186 kDa). |
|
IP
Use at 2-5 µg/mg of lysate. |
|
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Plays an essential role in cytokinesis. -
配列類似性
Belongs to the kinesin-like protein family.
Contains 1 FHA domain.
Contains 1 kinesin-motor domain. -
発生段階
Up-regulated in cells progressing through G2/M phase. -
細胞内局在
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Nuclear localization observed during interphase in PubMed:16431929 or triggered by entry into mitosis in PubMed:16648480. Cytoplasmic in interphase (PubMed:16648480) and metaphase cells (PubMed:16431929). From prophase to metaphase, accumulates at the developing spindle poles and their associated microtubules. During anaphase, accumulates at the spindle midzone. Localization to the central spindle and midbody during anaphase is dependent upon PRC1 and CIT presence. In cells ready to undergo abscission, concentrates at the contractile ring. - Information by UniProt
-
参照データベース
- Entrez Gene: 9928 Human
- Omim: 611279 Human
- SwissProt: Q15058 Human
- Unigene: 3104 Human
-
別名
- KIAA0042 antibody
- KIF 14 antibody
- KIF14 antibody
see all
画像
-
Western blot - Anti-KIF14 antibody (ab71155)All lanes : Anti-KIF14 antibody (ab71155) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 186 kDa
Observed band size: 210 kDa why is the actual band size different from the predicted?
Additional bands at: 180 kDa, 190 kDa, 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF14 antibody (ab71155)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling KIF14 with ab71155 at 1/200 (1µg/ml). Detection: DAB. -
Immunocytochemistry/ Immunofluorescence - Anti-KIF14 antibody (ab71155)Immunocytochemistry/Immunofluorescence analysis of formaldelyde-fixed HeLa cells labelling KIF14 with ab71155 at 1/100 (2µg/ml). A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody. -
Immunoprecipitation - Anti-KIF14 antibody (ab71155)Immunoprecipitation of HeLa whole cell lysate (1 mg) using ab71155 at 3 µg/mg lysate (lane 1) or control IgG (lane 2). 20% of immunoiprecipitate was analysed by Western blot using ab71155 at 1 µg/ml.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (7)
ab71155 は 7 報の論文で使用されています。
- Shou Y et al. Exosomes-derived miR-154-5p attenuates esophageal squamous cell carcinoma progression and angiogenesis by targeting kinesin family member 14. Bioengineered 13:4610-4620 (2022). PubMed: 35156510
- Pallavicini G et al. Goldberg-Shprintzen syndrome protein KIF1BP is a CITK interactor implicated in cytokinesis. J Cell Sci 134:N/A (2021). PubMed: 34100550
- Chen J et al. MiR-154-5p Suppresses Cell Invasion and Migration Through Inhibiting KIF14 in Nasopharyngeal Carcinoma. Onco Targets Ther 13:2235-2246 (2020). PubMed: 32214824
- Jungwirth G et al. Identification of KIF11 As a Novel Target in Meningioma. Cancers (Basel) 11:N/A (2019). PubMed: 30991738
- Tang X et al. The histone H3 lysine-27 demethylase UTX plays a critical role in colorectal cancer cell proliferation. Cancer Cell Int 19:144 (2019). PubMed: 31139021
- Xu H et al. Long non-coding RNA PAXIP1-AS1 facilitates cell invasion and angiogenesis of glioma by recruiting transcription factor ETS1 to upregulate KIF14 expression. J Exp Clin Cancer Res 38:486 (2019). PubMed: 31823805
- Huang W et al. Inhibition of KIF14 Suppresses Tumor Cell Growth and Promotes Apoptosis in Human Glioblastoma. Cell Physiol Biochem 37:1659-70 (2015). PubMed: 26536004