Anti-KIAA0528 抗体 (ab80470)
Key features and details
- Rabbit polyclonal to KIAA0528
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-KIAA0528 antibody -
製品の詳細
Rabbit polyclonal to KIAA0528 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Sea urchin, Orangutan, Xenopus tropicalis
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免疫原
Synthetic peptide within Human KIAA0528 aa 350-450. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_055617.1
Database link: Q86YS7 -
ポジティブ･コントロール
- HeLa whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab80470の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 110 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 110 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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関連性
KIAA0528 is a protein that was first identified in the in silico analysis of long cDNAs isolated in the Kazusa cDNA sequencing project. The function of KIAA0528 has not been characterized. By similarity KO528 contains a C2 domain, a domain that is found in proteins that bind phospholipids. The C2 domain is also present in a family of proteins involved in synaptic vesicle trafficking; thus, the function of KIAA0528 is predicted to be involved in intracellular transport. -
参照データベース
- Entrez Gene: 9847 Human
- SwissProt: Q86YS7 Human
- SwissProt: Q7TPS5 Mouse
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別名
- DKFZp779N2044 antibody
- Hypothetical protein LOC9847 antibody
- Uncharacterized protein KIAA0528 antibody
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIAA0528 antibody (ab80470)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse intestine tissue labelling KIAA0528 with ab80470 at 1/400 (2.5µg/ml). Detection: DAB.
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Western blot - Anti-KIAA0528 antibody (ab80470)All lanes : Anti-KIAA0528 antibody (ab80470) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 110 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted? -
Immunoprecipitation - Anti-KIAA0528 antibody (ab80470)Lane 1: ab80470, at 1 µg/mg, staining KIAA0528 in HeLa whole cell lysate by Western blot of immunoprecipitate (1 mg of lysate for IP, 20% of IP loaded). Immunoprecipitation was performed by using ab80470 at 3 µg/mg of lysate.
Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab80470 は論文での使用が確認できていません。