Anti-KHSRP 抗体 [EPR9864] (ab150393)
![Western blot - Anti-KHSRP antibody [EPR9864] (ab150393)](https://www.abcam.co.jp/ps/products/150/ab150393/Images/ab150393-157012-anti-khsrp-antibody-epr9864-western-blot.jpg "Western blot - Anti-KHSRP antibody [EPR9864] (ab150393)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9864] to KHSRP
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-KHSRP antibody [EPR9864]
KHSRP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9864] to KHSRP -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- 293T, HeLa, MCF7 and A673 cell lysates; Human brain and colon tissues; MCF7 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR9864 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab150393の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/250.
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| Flow Cyt (Intra) |
1/2000.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 73 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
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Flow Cyt (Intra)
1/2000. |
ターゲット情報
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機能
Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs. -
組織特異性
Detected in neural and non-neural cell lines. -
配列類似性
Belongs to the KHSRP family.
Contains 4 KH domains. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Cytoplasm. A small proportion is also found in the cytoplasm of neuronal cell bodies and dendrites. - Information by UniProt
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参照データベース
- Entrez Gene: 8570 Human
- Entrez Gene: 16549 Mouse
- Entrez Gene: 171137 Rat
- Omim: 603445 Human
- SwissProt: Q92945 Human
- SwissProt: Q3U0V1 Mouse
- SwissProt: Q99PF5 Rat
- Unigene: 726172 Human
see all -
別名
- Far upstream element-binding protein 2 antibody
- FBP2 antibody
- FUBP2 antibody
see all
画像
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Western blot - Anti-KHSRP antibody [EPR9864] (ab150393)All lanes : Anti-KHSRP antibody [EPR9864] (ab150393) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A673 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution
Predicted band size: 73 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9864] (ab150393)](https://www.abcam.co.jp/ps/products/150/ab150393/Images/ab150393-157011-anti-khsrp-antibody-epr9864-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9864] (ab150393)Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling KHSRP with ab150393 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-KHSRP antibody [EPR9864] (ab150393)](https://www.abcam.co.jp/ps/products/150/ab150393/Images/ab150393-281348-anti-khsrp-antibody-epr9864-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-KHSRP antibody [EPR9864] (ab150393)Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling KHSRP (red) with purified ab150393 at a 1/2000 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9864] (ab150393)](https://www.abcam.co.jp/ps/products/150/ab150393/Images/ab150393-157010-anti-khsrp-antibody-epr9864-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KHSRP antibody [EPR9864] (ab150393)Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling KHSRP with ab150393 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-KHSRP antibody [EPR9864] (ab150393)](https://www.abcam.co.jp/ps/products/150/ab150393/Images/ab150393-157009-anti-khsrp-antibody-epr9864-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-KHSRP antibody [EPR9864] (ab150393)Immunofluorescent analysis of MCF7 cells labelling KHSRP with ab150393 at 1/100 dilution. -
![Anti-KHSRP antibody [EPR9864] (ab150393)](https://www.abcam.co.jp/ps/products/150/ab150393/Images/ab150393-6-benefits-of-recombinant-antibodies.png)
Anti-KHSRP antibody [EPR9864] (ab150393)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab150393 は 6 報の論文で使用されています。
- Bao K et al. Fangchinoline suppresses conjunctival melanoma by directly binding FUBP2 and inhibiting the homologous recombination pathway. Cell Death Dis 12:380 (2021). PubMed: 33828201
- Mantini G et al. Co-expression analysis of pancreatic cancer proteome reveals biology and prognostic biomarkers. Cell Oncol (Dordr) 43:1147-1159 (2020). PubMed: 32860207
- Zhao H et al. KSRP specifies monocytic and granulocytic differentiation through regulating miR-129 biogenesis and RUNX1 expression. Nat Commun 8:1428 (2017). PubMed: 29127290
- Yuan X et al. USP39 promotes colorectal cancer growth and metastasis through the Wnt/ß-catenin pathway. Oncol Rep 37:2398-2404 (2017). PubMed: 28259917
- Pruksakorn D et al. Overexpression of KH-type splicing regulatory protein regulates proliferation, migration, and implantation ability of osteosarcoma. Int J Oncol 49:903-12 (2016). WB . PubMed: 27573585
- Li X et al. Suppression of HPV-16 late L1 5'-splice site SD3632 by binding of hnRNP D proteins and hnRNP A2/B1 to upstream AUAGUA RNA motifs. Nucleic Acids Res N/A:N/A (2013). WB ; Human . PubMed: 24013563