Anti-ketohexokinase 抗体 (ab154405)
Key features and details
- Rabbit polyclonal to ketohexokinase
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-ketohexokinase antibody
ketohexokinase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ketohexokinase -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
A recombinant protein fragment corresponding to a region within amino acids 1-248 of Human ketohexokinase (UniProt ID: P50053).
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ポジティブ･コントロール
- HeLa and HepG2 whole cell lysates; mouse liver tissue lysate; A549 xenograft
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154405の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tris-EDTA buffer (pH 8.0) can also be used.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 33 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tris-EDTA buffer (pH 8.0) can also be used. |
ターゲット情報
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組織特異性
Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye. -
パスウェイ
Carbohydrate metabolism; fructose metabolism. -
関連疾患
Defects in KHK are the cause of fructosuria (FRUCT) [MIM:229800]. Fructosuria is a benign defect of intermediary metabolism. -
配列類似性
Belongs to the carbohydrate kinase pfkB family. - Information by UniProt
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参照データベース
- Entrez Gene: 3795 Human
- Entrez Gene: 16548 Mouse
- Entrez Gene: 25659 Rat
- Omim: 614058 Human
- SwissProt: P50053 Human
- SwissProt: P97328 Mouse
- SwissProt: Q02974 Rat
- Unigene: 567297 Human
see all -
別名
- EC 2.7.1.3 antibody
- Fructokinase antibody
- Hepatic fructokinase antibody
see all
画像
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Western blot - Anti-ketohexokinase antibody (ab154405)Anti-ketohexokinase antibody (ab154405) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 33 kDa
12% SDS PAGE -
Western blot - Anti-ketohexokinase antibody (ab154405)Anti-ketohexokinase antibody (ab154405) at 1/10000 dilution + mouse liver tissue lysate at 20 µg
Predicted band size: 33 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ketohexokinase antibody (ab154405)Immunohistochemical analysis of paraffin-embedded A549 xenograft labeling ketohexokinase using ab154405 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab154405 は 2 報の論文で使用されています。
- Jeong S et al. High Fructose Drives the Serine Synthesis Pathway in Acute Myeloid Leukemic Cells. Cell Metab 33:145-159.e6 (2021). PubMed: 33357456
- Miller CO et al. Ketohexokinase knockout mice, a model for essential fructosuria, exhibit altered fructose metabolism and are protected from diet-induced metabolic defects. Am J Physiol Endocrinol Metab 315:E386-E393 (2018). PubMed: 29870677