AB154405

Anti-ketohexokinase 抗体

Name: Anti-ketohexokinase 抗体 (ab154405) | Abcam
Brand: Abcam Limited
SKU: AB154405
Price: 78000 JPY
Availability: InStock

Anti-ketohexokinase antibody

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(3 Publications)

Rabbit Polyclonal ketohexokinase antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Ketohexokinase aa 1-250.

別名を表示する

Ketohexokinase, Hepatic fructokinase, KHK

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ketohexokinase antibody (AB154405)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ketohexokinase antibody (AB154405)

Immunohistochemical analysis of paraffin-embedded A549 xenograft labeling ketohexokinase using ab154405 at 1/500 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ketohexokinase antibody (AB154405)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue stained for Ketohexokinase protein at cytoplasm with ab154405 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ketohexokinase antibody (AB154405)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue stained for Ketohexokinase protein at cytoplasm and nucleus with ab154405 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-ketohexokinase antibody (AB154405)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-ketohexokinase antibody (ab154405) at 1/1000 dilution

Lane 1:

HeLa whole cell extracts at 30 µg

Lane 2:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 33 kDa

false

Unknown

Western blot - Anti-ketohexokinase antibody (AB154405)

12% SDS PAGE

All lanes:

Western blot - Anti-ketohexokinase antibody (ab154405) at 1/10000 dilution

All lanes:

mouse liver tissue lysate at 20 µg

Predicted band size: 33 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human Ketohexokinase aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P50053

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Tris-EDTA buffer (pH 8.0) can also be used. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Tris-EDTA buffer (pH 8.0) can also be used. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Ketohexokinase also known as KHK or fructokinase is an enzyme that catalyzes the phosphorylation of fructose to fructose-1-phosphate utilizing ATP in the process. This enzyme has two isoforms: KHK-C and KHK-A with the cytosolic form (KHK-C) being approximately 35 kDa in mass. KHK is expressed at high levels in the liver kidney cortex and small intestine showing its importance for fructose metabolism in these tissues. The liver in particular exhibits extensive expression which facilitates the initial step in fructose metabolism.

Biological function summary

Ketohexokinase plays a significant role in the initial stage of fructose metabolism by converting fructose to fructose-1-phosphate. This reaction is a part of the fructose metabolic pathway enabling the use of fructose as an energy source especially in hepatocytes. KHK operates as a monomer and does not form part of any larger protein complex. The enzyme importantly controls the rate of fructose utilization preventing accumulation of free fructose which could lead to metabolic issues.

Pathways

Ketohexokinase belongs to the fructose metabolism pathway which is a subset of the larger carbohydrate metabolism. This enzyme's product fructose-1-phosphate subsequently interacts with aldolase B which is involved in further steps of the glycolysis and gluconeogenesis pathways. Besides aldolase B ketohexokinase's activity influences the balance between fructose and glucose metabolism connecting fructolysis with glycolytic and gluconeogenic pathways.

Dysfunction of ketohexokinase has been linked to hereditary fructose intolerance a condition resulting from a deficiency of aldolase B where the accumulation of fructose-1-phosphate can cause liver damage and hypoglycemia. KHK has also been implicated in metabolic syndrome where excessive fructose consumption exacerbates conditions like insulin resistance and fatty liver disease. The relationship of KHK with metabolic syndrome highlights its interaction with insulin pathways which involves proteins such as insulin receptor substrates that are affected by altered fructose metabolism.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.

See full target information Ketohexokinase

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Experimental dermatology 33:e15186 PubMed39367569

2024

Inhibition of Ubiquitin C-Terminal Hydrolase L1 Facilitates Cutaneous Wound Healing via Activating TGF-β/Smad Signalling Pathway in Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Huihui Pan,Jinru Song,Qing An,Junyi Chen,Wenyue Zheng,Litian Zhang,Jingjing Gu,Chengcheng Deng,Bin Yang

Cell metabolism 33:145-159.e6 PubMed33357456

2020

High Fructose Drives the Serine Synthesis Pathway in Acute Myeloid Leukemic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sangmoo Jeong,Angela Maria Savino,Rachel Chirayil,Ersilia Barin,Yuanming Cheng,Sun-Mi Park,Alexandra Schurer,Edouard Mullarky,Lewis C Cantley,Michael G Kharas,Kayvan R Keshari

American journal of physiology. Endocrinology and metabolism 315:E386-E393 PubMed29870677

2018

Ketohexokinase knockout mice, a model for essential fructosuria, exhibit altered fructose metabolism and are protected from diet-induced metabolic defects.

Applications

Unspecified application

Species

Unspecified reactive species

Corin O Miller,Xiaodong Yang,Ku Lu,Jin Cao,Kithsiri Herath,Thomas W Rosahl,Roger Askew,Guillaume Pavlovic,Gaochao Zhou,Cai Li,Taro E Akiyama

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ketohexokinase 抗体

Anti-ketohexokinase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ketohexokinase antibody (AB154405)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ketohexokinase antibody (AB154405)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ketohexokinase antibody (AB154405)

Western blot - Anti-ketohexokinase antibody (AB154405)

Western blot - Anti-ketohexokinase antibody (AB154405)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

Abcam product promise