AB2898

Anti-KDEL 抗体

Name: Anti-KDEL 抗体 (ab2898) | Abcam
Brand: Abcam Limited
SKU: AB2898
Rating: 4 (1 reviews)

Anti-KDEL antibody

(1 Review)

(7 Publications)

Rabbit Polyclonal KDEL antibody. Suitable for IHC-P, ICC/IF, Flow Cyt and reacts with Mouse, Human samples. Cited in 7 publications.

別名を表示する

Lys Asp Glu Leu

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (AB2898)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (AB2898)

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse liver tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1 : 100 with a rabbit polyclonal antibody recognizing KDEL ab2898 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (AB2898)

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse pancreas tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1 : 100 with a rabbit polyclonal antibody recognizing KDEL ab2898 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Immunocytochemistry/ Immunofluorescence - Anti-KDEL antibody (AB2898)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KDEL antibody (AB2898)

Immunofluorescent analysis of U-251 MG (Human brain glioma cell line) cells labeling KDEL (green) with ab2898. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2898 at 1/200 dilution overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (AB2898)

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse lymph node tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1 : 200 with a rabbit polyclonal antibody recognizing KDEL ab2898 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Flow Cytometry - Anti-KDEL antibody (AB2898)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-KDEL antibody (AB2898)

Flow cytometry analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling KDEL with ab2898 (purple) or a rabbit IgG isotype control (black) at a 10 μg/mL. After incubation for 1 hour on ice, the cells were labeled with a Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 647 conjugate at 1/50 dilution for 1 hour on ice. A representative 10,000 cells were acquired and analyzed for each sample.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

IHC-P, Flow Cyt, ICC/IF

applications

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-20 µg/mL", "FlowCyt-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

style

left-column

下記製品もご検討ください

AB240190

Anti-KDEL antibody [EPR12668] - BSA and Azide free

0 Reviews

製品ページを見る

こちらの製品も推奨する理由

私たちは、より高品質な製品をお届けするために、製品構成を常に改善しています。今回ご紹介する代替製品は、性能の向上、使いやすさや利便性の向上、またはより早くお届けできる点などを踏まえて選定しています。

style

left-column

製品の詳細

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

Preservative: 0.05% Sodium azide Constituents: 99% PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

See full target information KDEL

style

left-column

文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:297 PubMed37120609

2023

Perturbation of the host cell Ca homeostasis and ER-mitochondria contact sites by the SARS-CoV-2 structural proteins E and M.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Poggio,Francesca Vallese,Andreas J W Hartel,Travis J Morgenstern,Scott A Kanner,Oliver Rauh,Flavia Giamogante,Lucia Barazzuol,Kenneth L Shepard,Henry M Colecraft,Oliver Biggs Clarke,Marisa Brini,Tito Calì

Cells 11: PubMed35626680

2022

Stable Integration of Inducible SPLICS Reporters Enables Spatio-Temporal Analysis of Multiple Organelle Contact Sites upon Modulation of Cholesterol Traffic.

Applications

Unspecified application

Species

Unspecified reactive species

Flavia Giamogante,Lucia Barazzuol,Elena Poggio,Marta Tromboni,Marisa Brini,Tito Calì

The Journal of biological chemistry 298:101904 PubMed35398096

2022

Carbamazepine promotes surface expression of mutant Kir6.2-A28V ATP-sensitive potassium channels by modulating Golgi retention and autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ching-Han Lin,Yu-Chi Lin,Shi-Bing Yang,Pei-Chun Chen

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22228 PubMed35218571

2022

TIMP2 mediates endoplasmic reticulum stress contributing to sepsis-induced acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Nanhui Jiang,Rong Huang,Jiahao Zhang,Dongxue Xu,Tianlong Li,Zhongyi Sun,Lianjiu Su,Zhiyong Peng

The international journal of biochemistry & cell b 113:67-74 PubMed31185295

2019

N-Glycan-calnexin interactions in human factor VII secretion and deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wang,Lina Wang,Shuo Li,Ningzheng Dong,Qingyu Wu

Journal of proteomics 75:2855-68 PubMed22270009

2012

Proteomic analysis of human hepatoma cells expressing methionine adenosyltransferase I/III: Characterization of DDX3X as a target of S-adenosylmethionine.

Applications

Unspecified application

Species

Unspecified reactive species

Paul C Schröder,Joaquín Fernández-Irigoyen,Emilie Bigaud,Antonio Serna,Rubén Renández-Alcoceba,Shelly C Lu,José M Mato,Jesús Prieto,Fernando J Corrales

Molecular & cellular proteomics : MCP 10:M111.009126 PubMed21467216

2011

HSV-1 Cgal+ infection promotes quaking RNA binding protein production and induces nuclear-cytoplasmic shuttling of quaking I-5 isoform in human hepatoma cells.

Applications

Species

Unspecified reactive species

Virginia Sánchez-Quiles,María I Mora,Victor Segura,Anna Greco,Alberto L Epstein,Maria Giovanna Foschini,Loïc Dayon,Jean-Charles Sanchez,Jesús Prieto,Fernando J Corrales,Enrique Santamaría

View all publications

style

left-column

style

left-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

style

left-column

style

right-column

Anti-KDEL 抗体

Anti-KDEL antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (AB2898)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (AB2898)

Immunocytochemistry/ Immunofluorescence - Anti-KDEL antibody (AB2898)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (AB2898)

Flow Cytometry - Anti-KDEL antibody (AB2898)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

Reactivity data

下記製品もご検討ください

Anti-KDEL antibody [EPR12668] - BSA and Azide free

こちらの製品も推奨する理由

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (7)

Abcam product promise

関連製品

Alternative Product

Primary Antibodies

Alexa Fluor® 555 Anti-KDEL antibody [EPR12668] - ER Marker

Alternative Product

Primary Antibodies

Alexa Fluor® 488 Anti-KDEL antibody [EPR12668] - ER Marker

Alternative Product

Primary Antibodies

Anti-KDEL antibody [EPR12668]

Alternative Product

Primary Antibodies

Alexa Fluor® 647 Anti-KDEL antibody [EPR12668] - ER Marker