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リコンビナントRabMAb

Anti-KCTD9 抗体 [EPR13909] (ab180937)

Reviews (1) Submit a question References (2)
Western blot - Anti-KCTD9 antibody [EPR13909] (ab180937)
  • Immunocytochemistry/ Immunofluorescence - Anti-KCTD9 antibody [EPR13909] (ab180937)
  • Anti-KCTD9 antibody [EPR13909] (ab180937)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13909] to KCTD9
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-KCTD9 antibody [EPR13909]
    KCTD9 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13909] to KCTD9

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IFmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • MCF-7, HepG2, HT29 cell lysate; MCF-7 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab180937の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 43 kDa.
ICC/IF
1/50.
特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 43 kDa.
ICC/IF
1/50.

ターゲット情報

  • 関連性

    KCTD9 contains a potassium channel tetramerisation domain. The N-terminal, cytoplasmic tetramerisation domain (T1) of voltage-gated potassium channels encodes molecular determinants for subfamily-specific assembly of alpha-subunits into functional tetrameric channels. The specific function of KCTD9 is unknown.

  • 細胞内局在

    Cytoplasmic and Plasma membrane

  • 参照データベース

    • 別名

      • BTB/POZ domain containing protein KCTD9 antibody
      • BTBD27 antibody
      • FLJ20038 antibody
      • KCTD 9 antibody
      • Potassium channel tetramerisation domain containing 9 antibody
      see all

    画像

    • Western blot - Anti-KCTD9 antibody [EPR13909] (ab180937)
      Western blot - Anti-KCTD9 antibody [EPR13909] (ab180937)
      All lanes : Anti-KCTD9 antibody [EPR13909] (ab180937) at 1/50000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HT-29 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 43 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-KCTD9 antibody [EPR13909] (ab180937)
      Immunocytochemistry/ Immunofluorescence - Anti-KCTD9 antibody [EPR13909] (ab180937)

      Immunofluorescence analysis of MCF7 cells labeling KCTD9 using ab180937 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.

    • Anti-KCTD9 antibody [EPR13909] (ab180937)
      Anti-KCTD9 antibody [EPR13909] (ab180937)

    参考文献 (2)

    ab180937 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab180937 は 2 報の論文で使用されています。

    • Yao H  et al. KCTD9 inhibits the Wnt/β-catenin pathway by decreasing the level of β-catenin in colorectal cancer. Cell Death Dis 13:761 (2022). PubMed: 36055981
    • Hermann BP  et al. The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids. Cell Rep 25:1650-1667.e8 (2018). PubMed: 30404016

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Immunohistochemistry (Frozen sections) abreview for Anti-KCTD9 antibody [EPR13909]

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Testis, adult)
    Permeabilization
    Yes - 0.1% Triton X-100 in PBS
    Specification
    Testis, adult
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Bryan Niedenberger

    Verified customer

    投稿 Aug 22 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com