Anti-KCTD15 抗体 (ab106373)
Key features and details
- Rabbit polyclonal to KCTD15
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-KCTD15 antibody
KCTD15 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KCTD15 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human KCTD15 (C terminal).
Database link: Q96SI1 -
ポジティブ･コントロール
- Human spleen tissue lysate; Human spleen tissue; HeLa cell lysate
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特記事項
Antibodies should not be exposed to prolonged high temperatures.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Please see notes section. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab106373 is purified by affinity chromatography via a peptide column. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106373の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 2.5 - 20 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 32 kDa.
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| 特記事項 |
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IHC-P
Use a concentration of 2.5 - 20 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 32 kDa. |
ターゲット情報
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配列類似性
Contains 1 BTB (POZ) domain. - Information by UniProt
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参照データベース
- Entrez Gene: 79047 Human
- SwissProt: Q96SI1 Human
- Unigene: 221873 Human
- Unigene: 606506 Human
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別名
- BTB/POZ domain containing protein KCTD15 antibody
- BTB/POZ domain-containing protein kctd15 antibody
- KCD15_HUMAN antibody
see all
画像
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Western blot - Anti-KCTD15 antibody (ab106373)All lanes : Anti-KCTD15 antibody (ab106373) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate with Blocking buffer
Predicted band size: 32 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD15 antibody (ab106373)ab106373 at 2.5µg/ml staining KCTD15 in Human spleen by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD15 antibody (ab106373)Immunohistochemical analysis of human spleen tissue labelling KCTD15 with ab106373 at 20 ug/mL.
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Western blot - Anti-KCTD15 antibody (ab106373)Lane 1 : Anti-KCTD15 antibody (ab106373) at 0.5 µg/ml
Lane 2 : Anti-KCTD15 antibody (ab106373) at 1 µg/ml
All lanes : Human spleen tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 32 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab106373 は 3 報の論文で使用されています。
- Raymundo JR et al. KCTD1/KCTD15 complexes control ectodermal and neural crest cell functions, and their impairment causes aplasia cutis. J Clin Invest 134:N/A (2023). PubMed: 38113115
- Coppola L et al. KCTD15 Is Overexpressed in her2+ Positive Breast Cancer Patients and Its Silencing Attenuates Proliferation in SKBR3 CELL LINE. Diagnostics (Basel) 12:N/A (2022). PubMed: 35328144
- Smaldone G et al. The essential player in adipogenesis GRP78 is a novel KCTD15 interactor. Int J Biol Macromol 115:469-475 (2018). WB, IP ; Mouse . PubMed: 29665387