Anti-KCC1 抗体 (ab115607)
Key features and details
- Goat polyclonal to KCC1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-KCC1 antibody -
製品の詳細
Goat polyclonal to KCC1 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig
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免疫原
Synthetic peptide corresponding to Human KCC1 aa 950-1050 (internal sequence) (Cysteine residue).
Database link: NP_005063.1 -
ポジティブ･コントロール
- Human Placenta tissue; Human heart lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115607の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 121 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 121 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl(-) exit in NaCl absorbing epithelia (By similarity). Isoform 4 has no transport activity. -
組織特異性
Ubiquitous. Levels are much higher in erythrocytes from patients with Hb SC and Hb SS compared to normal AA erythrocytes. This may contribute to red blood cell dehydration and to the manifestation of sickle cell disease by increasing the intracellular concentration of HbS. Isoform 1 was not detected in circulating reticulocytes. -
配列類似性
Belongs to the SLC12A transporter family. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6560 Human
- Entrez Gene: 20498 Mouse
- Entrez Gene: 396992 Pig
- Entrez Gene: 29501 Rat
- Omim: 604119 Human
- SwissProt: Q9UP95 Human
- SwissProt: Q9JIS8 Mouse
- SwissProt: Q28677 Rabbit
see all -
別名
- Electroneutral potassium chloride cotransporter 1 antibody
- Electroneutral potassium-chloride cotransporter 1 antibody
- Erythroid K Cl cotransporter 1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCC1 antibody (ab115607)
ab115607, at 5µg/ml, staining KCC1 in Formalin-fixed, Paraffin-embedded Human Placenta tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Western blot - Anti-KCC1 antibody (ab115607)Anti-KCC1 antibody (ab115607) at 0.3 µg/ml + Human heart lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 121 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab115607 は 1 報の論文で使用されています。
- Steffensen AB et al. Cotransporter-mediated water transport underlying cerebrospinal fluid formation. Nat Commun 9:2167 (2018). PubMed: 29867199