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Anti-KAT7 / Hbo1 / MYST2 抗体 (ab70183)

Submit a review Q&A (1)References (14)
Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183)
  • Immunocytochemistry/ Immunofluorescence - Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183)

Key features and details

  • Rabbit polyclonal to KAT7 / Hbo1 / MYST2
  • Suitable for: ELISA, IHC-P, WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-KAT7 / Hbo1 / MYST2 antibody
    KAT7 / Hbo1 / MYST2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to KAT7 / Hbo1 / MYST2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ELISA, IHC-P, WB, ICC/IF, IPmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide corresponding to Human KAT7/ Hbo1/ MYST2 (internal sequence).
    Database link: O95251
    (Peptide available as ab182013)

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab70183の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/500 - 1/1000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
ICC/IF
1/100 - 1/500.
IP
Use at an assay dependent concentration.
特記事項
ELISA
Use at an assay dependent concentration.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/500 - 1/1000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
ICC/IF
1/100 - 1/500.
IP
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Specifically represses AR-mediated transcription.

  • 組織特異性

    Ubiquitously expressed, with highest levels in testis.

  • 配列類似性

    Belongs to the MYST (SAS/MOZ) family.
    Contains 1 C2HC-type zinc finger.

  • ドメイン

    The C2HC-type zinc finger is required for interaction with MCM2 and ORC1L.
    The N-terminus is involved in transcriptional repression, while the C-terminus mediates AR-interaction.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Nucleus > nucleoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Hbo 1 antibody
    • HBO1 antibody
    • HBOa antibody
    • Histone acetyltransferase binding to hORC1 antibody
    • Histone acetyltransferase binding to ORC1 antibody
    • Histone acetyltransferase KAT7 antibody
    • Histone acetyltransferase MYST2 antibody
    • K(lysine) acetyltransferase 7 antibody
    • KAT 7 antibody
    • KAT7 antibody
    • Lysine acetyltransferase 7 antibody
    • MOZ antibody
    • MOZ YBF2/SAS3 SAS2 and TIP60 protein antibody
    • MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2 antibody
    • MYST 2 antibody
    • MYST histone acetyltransferase 2 antibody
    • MYST protein 2 antibody
    • MYST-2 antibody
    • MYST2 antibody
    • MYST2_HUMAN antibody
    • SAS 2 antibody
    • SAS2 and TIP60 protein 2 antibody
    • TIP60 protein 2 antibody
    • YBF2/SAS3 antibody
    • ZC2HC7 antibody
    see all

画像

  • Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183)
    Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183)
    All lanes : Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 71 kDa
    Observed band size: 71 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183)
    Immunocytochemistry/ Immunofluorescence - Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183)
    HUVEC cells with ab70183 at 1/500 dilution, with and without the immunising peptide.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183)
    IHC image of ab70183 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab70183, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献 (14)

ab70183 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab70183 は 14 報の論文で使用されています。

  • Zhang FF  et al. The circular RNA Rap1b promotes Hoxa5 transcription by recruiting Kat7 and leading to increased Fam3a expression, which inhibits neuronal apoptosis in acute ischemic stroke. Neural Regen Res 18:2237-2245 (2023). PubMed: 37056143
  • Xie M  et al. Sublytic C5b-9 induces glomerular mesangial cell proliferation via ERK1/2-dependent SOX9 phosphorylation and acetylation by enhancing Cyclin D1 in rat Thy-1 nephritis. Exp Mol Med 53:572-590 (2021). PubMed: 33811247
  • MacPherson L  et al. HBO1 is required for the maintenance of leukaemia stem cells. Nature 577:266-270 (2020). PubMed: 31827282
  • Khan O  et al. TOX transcriptionally and epigenetically programs CD8+ T cell exhaustion. Nature 571:211-218 (2019). PubMed: 31207603
  • Long C  et al. LPS promotes HBO1 stability via USP25 to modulate inflammatory gene transcription in THP-1 cells. Biochim Biophys Acta Gene Regul Mech 1861:773-782 (2018). PubMed: 30745998
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

Question

might test ab31506 in xenopus, send testing discount code

xenopus: NP001079995, check homology
check homology of other Abs as well

Read More
Answer

Thank you for your phone call.
The overall homology between Human and Xenopus is 93%. Thus, in general there is good chance for an antibody raised against human to work in Xenopus.
For ab31506 the homology of the immunogen with Xenopus is 100%.
For ab87100 the homology of the immunogen with Xenopus is 90%. For the other Hbo1 antibodies I currently do not have information about the immunogen range. I can check with the lab if you like - please let me know.
None of these antibodies has been tested in IP.
DISCOUNT CODE: xxx
Expiration date: xxx
I am very pleased to hear you would like to accept our offer and test ab31506 in Xenopus and IP. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for Xenopus/IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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