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AB246957

Anti-KAT3 抗体

Anti-KAT3 antibody

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(1 Publication)

Rabbit Polyclonal KAT3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KYAT3 aa 350 to C-terminus.

別名を表示する

CCBL2, KAT3, KYAT3, Kynurenine--oxoglutarate transaminase 3, Cysteine-S-conjugate beta-lyase 2, Kynurenine aminotransferase 3, Kynurenine aminotransferase III, Kynurenine--glyoxylate transaminase, Kynurenine--oxoglutarate transaminase III, KATIII

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT3 antibody (AB246957)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT3 antibody (AB246957)

Paraffin-embedded human testis tissue stained for KAT3 using ab246957 at 1/1000 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-KAT3 antibody (AB246957)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KAT3 antibody (AB246957)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for KAT3 (green) using ab246957 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT3 antibody (AB246957)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT3 antibody (AB246957)

Paraffin-embedded human placenta tissue stained for KAT3 using ab246957 at 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT3 antibody (AB246957)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT3 antibody (AB246957)

Paraffin-embedded human thyroid gland tissue stained for KAT3 using ab246957 at 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT3 antibody (AB246957)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT3 antibody (AB246957)

Paraffin-embedded human endometrium tissue stained for KAT3 using ab246957 at 1/1000 dilution in immunohistochemical analysis.

Western blot - Anti-KAT3 antibody (AB246957)
  • WB

Unknown

Western blot - Anti-KAT3 antibody (AB246957)

All lanes:

Western blot - Anti-KAT3 antibody (ab246957) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) whole cell lysate

Lane 3:

Human plasma

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 51 kDa

false

Western blot - Anti-KAT3 antibody (AB246957)
  • WB

Unknown

Western blot - Anti-KAT3 antibody (AB246957)

All lanes:

Western blot - Anti-KAT3 antibody (ab246957) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lane 2:

NBT-II (rat Wistar bladder tumor cell line) whole cell lysate

Predicted band size: 51 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

ICC/IF, WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human KYAT3 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6YP21

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

KAT3 also known as Cbp/p300 is a lysine acetyltransferase enzyme which plays a significant role in chromatin remodeling and transcriptional regulation by acetylating histones and non-histone proteins. Its molecular mass is approximately 265 kDa. This enzyme is expressed in a wide range of tissues indicating its participation in various cellular functions. KAT3’s widespread expression supports its involvement in multiple physiological processes critical to cell survival and differentiation.
Biological function summary

KAT3 functions as a transcriptional coactivator and is part of several transcriptional complexes. It interacts with various transcription factors enhancing their ability to regulate gene expression. By acetylating target nuclear proteins KAT3 modulates chromatin structure thereby facilitating gene transcription. This protein manipulation enables cell proliferation differentiation and maintenance of homeostasis highlighting its diverse biological roles.

Pathways

KAT3 actively participates in the signaling pathways RAS/RAF/MEK/ERK and PI3K/AKT illustrating its influence in cell growth and survival. It frequently interacts with proteins such as CREB and AP-1 which are vital in activating transcription and response to environmental signals. KAT3’s integration into these pathways highlights its critical involvement in managing cellular processes responsive to extracellular cues.

KAT3 is closely linked to cancer and Rubinstein-Taybi syndrome. Mutations or deregulation in KAT3 expression or function often result in abnormal cell growth contributing to oncogenesis. In Rubinstein-Taybi syndrome genetic mutations affecting KAT3 hinder normal developmental processes. Throughout these conditions KAT3 interacts with p53 a pivotal protein in tumor suppression and cell cycle control accentuating its relevance in maintaining cellular integrity and preventing disease progression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA), an intermediate in the tryptophan catabolic pathway which is also a broad spectrum antagonist of the three ionotropic excitatory amino acid receptors among others. May catalyze the beta-elimination of S-conjugates and Se-conjugates of L-(seleno)cysteine, resulting in the cleavage of the C-S or C-Se bond. Has transaminase activity towards L-kynurenine, tryptophan, phenylalanine, serine, cysteine, methionine, histidine, glutamine and asparagine with glyoxylate as an amino group acceptor (in vitro). Has lower activity with 2-oxoglutarate as amino group acceptor (in vitro).
See full target information KYAT3
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 20:114-130 PubMed37615625

2023

Tumor-derived lactate promotes resistance to bevacizumab treatment by facilitating autophagy enhancer protein RUBCNL expression through histone H3 lysine 18 lactylation (H3K18la) in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Weihao Li,Chi Zhou,Long Yu,Zhenlin Hou,Huashan Liu,Lingheng Kong,Yanbo Xu,Jiahua He,Jin Lan,Qingjian Ou,Yujing Fang,Zhenhai Lu,Xiaojun Wu,Zhizhong Pan,Jianhong Peng,Junzhong Lin
View all publications
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