Anti-KAT13D / CLOCK 抗体

• WB

Supplier Data

Western blot - Anti-KAT13D / CLOCK antibody (AB3517)

Electrophoresis performed on a 4-12% BisTris gel and proteins transferred onto a nitrocellulose membrane.

All lanes:

Western blot - Anti-KAT13D / CLOCK antibody (ab3517) at 1/2000 dilution

Lane 1:

Mouse skeletal muscle tissue at 30 µg

Lane 2:

Mouse liver tissue at 30 µg

Secondary

Lane 1:

Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution

Lane 2:

oat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution

Predicted band size: 95 kDa

true

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-KAT13D / CLOCK antibody (AB3517)

Immunocytochemistry/immunofluorescence analysis of U251 cells labeling KAT13D/CLOCK (green) with ab3517 at 1/100. Cells were fixed with formalin and permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blcoked with £% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. F-actin (red) was stained with phalloidin and nuclei (blue) were stained with Hoechst or DAPI. 60X magnification. Left - negative control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT13D / CLOCK antibody (AB3517)

ab3517 labelling KAT13D in the nucleus and cytoplasm of Human colon tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Thissue sections were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT13D / CLOCK antibody (AB3517)

ab3517 labelling KAT13D in the nucleus and cytoplasm of Human skeletal muscle tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Thissue sections were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT13D / CLOCK antibody (AB3517)

ab3517 labelling KAT13D in the nucleus and cytoplasm of Mouse colon tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Thissue sections were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

• IHC-P

AbReview18129****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT13D / CLOCK antibody (AB3517)

ab3517 staining KAT13D/CLOCK in Mouse skeletal muscle tissue sections by IHC-P (Paraformaldehyde-fixed, paraffin-embedded tissue sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in citrate buffer pH6. Samples were incubated with primary antibody (1/400 in PBS) for 12 hours at 4°C. Undiluted ab64256 was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

• WB

Supplier Data

Western blot - Anti-KAT13D / CLOCK antibody (AB3517)

All lanes:

Western blot - Anti-KAT13D / CLOCK antibody (ab3517) at 1/4000 dilution

Lane 1:

HeLa cell lysate at 25 µg

Lane 2:

NIH-3T3 cell lysate at 25 µg

Predicted band size: 95 kDa

Observed band size: 100 kDa

false

• WB

CiteAb

Western blot - Anti-KAT13D / CLOCK antibody (AB3517)

KAT13D / CLOCK western blot using anti-KAT13D / CLOCK antibody ab3517. Publication image and figure legend from Yao, Y., Ying, Y., et al., 2020, Front Physiol, PubMed 32390857.

ab3517 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab3517 please see the product overview.

A 40-Hz light flicker restores the expression levels of key players of central clock in the SCN of APP/PS1 mice. (A) mRNA expression of Bmal1, Clock, and Per2 in the SCN from control and APP/PSI mice with or without light exposure (n = 6). (B) Representative western blot showing protein expression levels of BMAL1, CLOCK, and PER2 of hypothalamus of mice with or without light exposure. Quantification was determined by five independent experiments. #p < 0.005, ##p < 0.01, ###p < 0.001 significantly different from the control group; *p < 0.005, ***p < 0.001, significantly different from the APP/PS1 group.

false