Anti-KAT13D / CLOCK 抗体 (ab3517)
Key features and details
- Rabbit polyclonal to KAT13D / CLOCK
- Suitable for: IHC-P, ICC/IF, IHC-Fr, WB, ChIP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-KAT13D / CLOCK antibody
KAT13D / CLOCK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KAT13D / CLOCK -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, IHC-Fr, WB, ChIPmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Mouse KAT13D/ CLOCK aa 1-100.
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ポジティブ･コントロール
- WB: human HeLa, mouse NIH-3T3 and skeletal muscle tissue; IHC: human skeletal muscle, colon tissue, mouse colon tissue; ICC: human U251 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3517の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
1/100 - 1/1000.
Immunohistochemical staining of CLOCK in hamster brain results in the staining of the superchiasmatic nucleus. |
| ICC/IF | (1) |
1/10 - 1/200.
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| EMSA |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| Gel Shift Assay |
Use at an assay dependent concentration.
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| WB | (3) |
1/200 - 1/2000. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).
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| ChIP |
Use at an assay dependent concentration. PubMed: 20956306
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| 特記事項 |
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IHC-P
1/100 - 1/1000. Immunohistochemical staining of CLOCK in hamster brain results in the staining of the superchiasmatic nucleus. |
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ICC/IF
1/10 - 1/200. |
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EMSA
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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Gel Shift Assay
Use at an assay dependent concentration. |
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WB
1/200 - 1/2000. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa). |
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ChIP
Use at an assay dependent concentration. PubMed: 20956306 |
ターゲット情報
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機能
ARNTL/2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression (By similarity). Acetylates primarily histones H3 and H4 (By similarity). Acetylates also a non-histone substrate: ARNTL. -
組織特異性
Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN). -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
翻訳後修飾
Phosphorylation is dependent on CLOCK-ARNTL heterodimer formation. -
細胞内局在
Cytoplasm. Nucleus. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is ARNTL-dependent. Phosphorylated form located in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 9575 Human
- Entrez Gene: 12753 Mouse
- Omim: 601851 Human
- SwissProt: O15516 Human
- SwissProt: O08785 Mouse
- Unigene: 436975 Human
- Unigene: 3552 Mouse
- Unigene: 392894 Mouse
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別名
- bHLHe8 antibody
- Circadian locomoter output cycles kaput protein antibody
- Circadian locomoter output cycles protein kaput antibody
see all
画像
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Western blot - Anti-KAT13D / CLOCK antibody (ab3517)All lanes : Anti-KAT13D / CLOCK antibody (ab3517) at 1/2000 dilution
Lane 1 : Mouse skeletal muscle tissue
Lane 2 : Mouse liver tissue
Lysates/proteins at 30 µg per lane.
Secondary
Lane 1 : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
Lane 2 : oat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 95 kDaElectrophoresis performed on a 4-12% BisTris gel and proteins transferred onto a nitrocellulose membrane.
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Immunocytochemistry/ Immunofluorescence - Anti-KAT13D / CLOCK antibody (ab3517)Immunocytochemistry/immunofluorescence analysis of U251 cells labeling KAT13D/CLOCK (green) with ab3517 at 1/100. Cells were fixed with formalin and permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blcoked with £% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. F-actin (red) was stained with phalloidin and nuclei (blue) were stained with Hoechst or DAPI. 60X magnification. Left - negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT13D / CLOCK antibody (ab3517)ab3517 labelling KAT13D in the nucleus and cytoplasm of Human skeletal muscle tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Thissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT13D / CLOCK antibody (ab3517)ab3517 labelling KAT13D in the nucleus and cytoplasm of Mouse colon tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Thissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT13D / CLOCK antibody (ab3517)ab3517 labelling KAT13D in the nucleus and cytoplasm of Human colon tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Thissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT13D / CLOCK antibody (ab3517)This image is courtesy of an anonymous Abreviewab3517 staining KAT13D/CLOCK in Mouse skeletal muscle tissue sections by IHC-P (Paraformaldehyde-fixed, paraffin-embedded tissue sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in citrate buffer pH6. Samples were incubated with primary antibody (1/400 in PBS) for 12 hours at 4°C. Undiluted ab64256 was used as the secondary antibody. -
Western blot - Anti-KAT13D / CLOCK antibody (ab3517)All lanes : Anti-KAT13D / CLOCK antibody (ab3517) at 1/4000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : NIH-3T3 cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 95 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (49)
ab3517 は 49 報の論文で使用されています。
- Niu L et al. Chronic sleep deprivation altered the expression of circadian clock genes and aggravated Alzheimer's disease neuropathology. Brain Pathol 32:e13028 (2022). PubMed: 34668266
- Cheng Q et al. miR-455-5p regulates circadian rhythms by accelerating the degradation of Clock mRNA. IUBMB Life 74:245-258 (2022). PubMed: 34904778
- Huang Y et al. miR-92b-3p Exerts Neuroprotective Effects on Ischemia/Reperfusion-Induced Cerebral Injury via Targeting NOX4 in a Rat Model. Oxid Med Cell Longev 2022:3494262 (2022). PubMed: 35401931
- Xuan W et al. Circadian Regulator CLOCK Drives Immunosuppression in Glioblastoma. Cancer Immunol Res 10:770-784 (2022). PubMed: 35413115
- Wang F et al. The Clock gene regulates kainic acid-induced seizures through inhibiting ferroptosis in mice. J Pharm Pharmacol 74:1640-1650 (2022). PubMed: 35704277