Anti-KAT13D / CLOCK 抗体 (ab178525)
Key features and details
- Goat polyclonal to KAT13D / CLOCK
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
製品の概要
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製品名
Anti-KAT13D / CLOCK antibody
KAT13D / CLOCK 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to KAT13D / CLOCK -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Chicken, Human, Orangutan
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免疫原
Synthetic peptide corresponding to Human KAT13D/ CLOCK aa 458-472 (internal sequence) (Cysteine residue). (NP_004889.1)
Sequence:DTSTPPRQHLPAHEK
Database link: O15516 -
ポジティブ･コントロール
- Mouse and rat skeletal lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab178525 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab178525の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 95 kDa.
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 95 kDa. 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
ARNTL/2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression (By similarity). Acetylates primarily histones H3 and H4 (By similarity). Acetylates also a non-histone substrate: ARNTL. -
組織特異性
Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN). -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
翻訳後修飾
Phosphorylation is dependent on CLOCK-ARNTL heterodimer formation. -
細胞内局在
Cytoplasm. Nucleus. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is ARNTL-dependent. Phosphorylated form located in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 373991 Chicken
- Entrez Gene: 9575 Human
- Entrez Gene: 12753 Mouse
- Entrez Gene: 100172630 Orangutan
- Entrez Gene: 60447 Rat
- Omim: 601851 Human
- SwissProt: Q8QGQ6 Chicken
- SwissProt: O15516 Human
see all -
別名
- bHLHe8 antibody
- Circadian locomoter output cycles kaput protein antibody
- Circadian locomoter output cycles protein kaput antibody
see all
画像
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Western blot - Anti-KAT13D / CLOCK antibody (ab178525)All lanes : Anti-KAT13D / CLOCK antibody (ab178525) at 0.03 µg/ml
Lane 1 : Mouse skeletal muscle lysate
Lane 2 : Rat skeletal muscle lysate
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 95 kDa
データシートおよび資料
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Datasheet download
参考文献 (0)
ab178525 は論文での使用が確認できていません。
