Anti-KAT13C / NCOA2 抗体 (ab10491)
Key features and details
- Rabbit polyclonal to KAT13C / NCOA2
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-KAT13C / NCOA2 antibody
KAT13C / NCOA2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KAT13C / NCOA2 -
由来種
Rabbit -
特異性
We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
アプリケーション
適用あり: WB, IHC-P, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Gorilla, Chinese hamster
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免疫原
Synthetic peptide corresponding to Human KAT13C/ NCOA2 aa 700-800.
Database link: Q15596 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Antibodies were affinity purified using the peptide immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10491の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 175 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| IP | (1) |
Use 6µg for 10 cells.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 175 kDa). |
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IHC-P
Use at an assay dependent concentration. |
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IP
Use 6µg for 10 cells. |
ターゲット情報
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機能
Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. -
関連疾患
Note=Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias. Inversion inv(8)(p11;q13) generates the KAT6A-NCOA2 oncogene, which consists of the N-terminal part of KAT6A and the C-terminal part of NCOA2/TIF2. KAT6A-NCOA2 binds to CREBBP and disrupts its function in transcription activation. -
配列類似性
Belongs to the SRC/p160 nuclear receptor coactivator family.
Contains 1 bHLH (basic helix-loop-helix) domain.
Contains 1 PAS (PER-ARNT-SIM) domain. -
ドメイン
Contains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant.
The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.
Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 10499 Human
- Entrez Gene: 17978 Mouse
- Entrez Gene: 83724 Rat
- Omim: 601993 Human
- SwissProt: Q15596 Human
- SwissProt: Q61026 Mouse
- SwissProt: Q9WUI9 Rat
- Unigene: 446678 Human
see all -
別名
- bHLHe75 antibody
- Class E basic helix-loop-helix protein 75 antibody
- glucocorticoid receptor interacting protein 1 antibody
see all
画像
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Western blot - Anti-KAT13C / NCOA2 antibody (ab10491)All lanes : Anti-KAT13C / NCOA2 antibody (ab10491) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : NCOA2 knockout HEK-293T cell lysate
Lane 3 : PC-3 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 175 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-KAT13C / NCOA2 antibody staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab10491 was shown to bind specifically to KAT13C / NCOA2. A band was observed at 160 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in NCOA2 knockout cell line ab265068 (knockout cell lysate ab258530). To generate this image, wild-type and NCOA2 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Immunoprecipitation - Anti-KAT13C / NCOA2 antibody (ab10491)ab10491 used for WB at 0.1 µg/ml (A)
Lane 1: Whole cell lysate Hela, 50 ug
Lane 2: Whole cell lysate Hela, 15 ug
Lane 3: Whole cell lysate HEK293T, 50 ug
Lane 4: Whole cell lysate Jurkat, 50 ug
ab10491 was used at 1 µg/ml for WB and used for IP at 6 µg/mg lysate (B)
Lane 1-3: ab10491]
Lane4: Control IgG
Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
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Western blot - Anti-KAT13C / NCOA2 antibody (ab10491)All lanes : Anti-KAT13C / NCOA2 antibody (ab10491) at 1 µg/ml
Lane 1 : TCMK-1 cells
Lane 2 : 4T1 cells
Lane 3 : CT26.WT cells
Lysates/proteins at 50 µg per lane.
Predicted band size: 175 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab10491 は 13 報の論文で使用されています。
- Mimouna S et al. Transcription cofactor GRIP1 differentially affects myeloid cell-driven neuroinflammation and response to IFN-ß therapy. J Exp Med 218:N/A (2021). PubMed: 33045064
- Yang Y et al. Hypothalamic steroid receptor coactivator-2 regulates adaptations to fasting and overnutrition. Cell Rep 37:110075 (2021). PubMed: 34879284
- Yao C et al. Thevebioside, the active ingredient of traditional Chinese medicine, promotes ubiquitin-mediated SRC-3 degradation to induce NSCLC cells apoptosis. Cancer Lett 493:167-177 (2020). PubMed: 32829007
- Escoter-Torres L et al. Anti-inflammatory functions of the glucocorticoid receptor require DNA binding. Nucleic Acids Res 48:8393-8407 (2020). PubMed: 32619221
- Rollins DA et al. Glucocorticoid-induced phosphorylation by CDK9 modulates the coactivator functions of transcriptional cofactor GRIP1 in macrophages. Nat Commun 8:1739 (2017). PubMed: 29170386