AB124727

Anti-Kappa light chain 抗体 [EPR5367-8]

Anti-Kappa light chain antibody [EPR5367-8]

BOND RX™ Validated
RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal IGKC antibody. Suitable for IHC-P, IP, ELISA, WB and reacts with Human samples. Cited in 5 publications.

別名を表示する

Immunoglobulin kappa constant, Ig kappa chain C region, Ig kappa chain C region AG, Ig kappa chain C region CUM, Ig kappa chain C region EU, Ig kappa chain C region OU, Ig kappa chain C region ROY, Ig kappa chain C region TI, IGKC

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5367-8] (AB124727)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5367-8] (AB124727)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Kappa light chain with purified ab124727 at 1/5000 dilution (0.06 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Lab

Immunoprecipitation - Anti-Kappa light chain antibody [EPR5367-8] (AB124727)

Purified ab124727 at 1/50 dilution (2μg) immunoprecipitating Kappa light chain in Human spleen lysate.
Lane 1 (input) : Human spleen lysate 10μg
Lane 2 (+) : ab124727 + Human spleen lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab124727 in Human spleen lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 25 kDa

All lanes:

Immunoprecipitation - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)

Predicted band size: 25 kDa

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ELISA - Anti-Kappa light chain antibody [EPR5367-8] (AB124727)

ELISA

Lab

ELISA - Anti-Kappa light chain antibody [EPR5367-8] (AB124727)

ELISA analysis of Kappa Light Chains, Lambda Light Chains, Human IgA, Human IgM, Human IgG, Rat IgG,and Mouse IgG at 1000ng/mL using purified ab124727. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at 1/2500 dilution.

Unknown

Western blot - Anti-Kappa light chain antibody [EPR5367-8] (AB124727)

All lanes:

Western blot - Anti-Kappa light chain antibody [EPR5367-8] (ab124727) at 1/5000 dilution

Lane 1:

Human tonsil lysate at 15 µg

Lane 2:

Human plasma lysate at 15 µg

Lane 3:

Human spleen lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 25 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR5367-8

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ELISA, IP, WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "antigen retrieval is recommended.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/16000", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The kappa light chain alternatively known as Ig kappa chain is a component of immunoglobulins which are important for immune response. This protein has a molecular mass of roughly 25 kDa. It predominantly expresses in B lymphocytes where it pairs with heavy chains to form antibodies. Its expression is indicative of mature B cell functions serving as a marker for certain types of immune cells. The kappa light chain can also be detected outside of cells circulating in the blood and other bodily fluids.

Biological function summary

Kappa light chains are integral to the structure and function of antibodies. As part of the immunoglobulin molecule these chains contribute to antigen specificity by combining with heavy chains. Together they form the antigen-binding site. The kappa light chain is not part of a multiprotein complex; rather it directly participates in the antibody response. The presence of either kappa or lambda light chains can determine the particularities of antibody characteristics.

Pathways

Kappa light chains are elements of the immunological pathways notably the adaptive immune response. They interact with other proteins like immunoglobulin heavy chains to neutralize pathogens. The constant region of the kappa chain plays a role in determining the isotype of immunoglobulins which affects how antibodies mediate immune effector functions. Cross-linking of surface immunoglobulin by antigens can trigger B cell activation an essential step in the adaptive immune system.

Kappa light chains associate closely with multiple myeloma and certain types of lymphomas. Overproduction of kappa light chains in these diseases results in high levels detectable in the serum or urine which can be used diagnostically as markers. Also dysregulation of kappa chains correlates with autoimmune disorders whereby the immune system incorrectly targets its tissues. In disorders like these the interplay between kappa and lambda chains often arises showing imbalance between the two as a sign of abnormal B cell activity.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).

See full target information IGKC

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1636162 PubMed41019035

2025

Monoclonal immunoglobulins on red blood cells: a potential supplementary diagnostic indicator for monoclonal gammopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Benxia Bing,Xiaowei Yang,Chenghua Wang,Liang Xu,Shiqing Cheng,Yun Liu,Qiuhan Wang,Chunjuan Zhai,Bing Zhao,Rong Wang,Jing Sun

Journal of cancer research and clinical oncology 150:299 PubMed38850382

2024

Effect of human bone marrow mesenchymal stem cell-derived microvesicles on the apoptosis of the multiple myeloma cell line U266.

Applications

Unspecified application

Species

Unspecified reactive species

Mona Vafaeizadeh,Saeid Abroun,Mina Soufi Zomorrod

Frontiers in endocrinology 13:1029297 PubMed36387920

2022

Development of a novel diagnostic assay for insulin receptor autoantibodies based on a patient with autoimmune hypoglycaemia.

Applications

Unspecified application

Species

Unspecified reactive species

Leiluo Geng,Cheuk-Lik Wong,Boya Liao,Ying Lin,Hao Han,Karen S L Lam,Aimin Xu,Chi-Ho Lee,Vicki H K Tam

Nature biomedical engineering 6:1248-1256 PubMed36138193

2022

A humanized minipig model for the toxicological testing of therapeutic recombinant antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana Flisikowska,Jerome Egli,Krzysztof Flisikowski,Marlene Stumbaum,Erich Küng,Martin Ebeling,Roland Schmucki,Guy Georges,Thomas Singer,Mayuko Kurome,Barbara Kessler,Valeri Zakhartchenko,Eckhard Wolf,Felix Weber,Angelika Schnieke,Antonio Iglesias

Molecular medicine reports 23: PubMed33760139

2021

Expression of immunoglobulin G in human proximal tubular epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenling Deng,Ziyang Jing,Yanhong Guo,Junfan Ma,Huige Yan,Zhan Shi,Hui Deng,Yaoxian Liang,Song Wang,Zhuan Cui,Yuejuan Pan,Xiaoyan Qiu,Yue Wang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Kappa light chain 抗体 [EPR5367-8]

Anti-Kappa light chain antibody [EPR5367-8]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5367-8] (AB124727)

Immunoprecipitation - Anti-Kappa light chain antibody [EPR5367-8] (AB124727)

ELISA - Anti-Kappa light chain antibody [EPR5367-8] (AB124727)

Western blot - Anti-Kappa light chain antibody [EPR5367-8] (AB124727)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (5)

Abcam product promise