JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB272068

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal KAP1 phospho S824 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TRIM28 pS824.

別名を表示する

KAP1, RNF96, TIF1B, TRIM28, Transcription intermediary factor 1-beta, TIF1-beta, E3 SUMO-protein ligase TRIM28, KRAB-associated protein 1, KRAB-interacting protein 1, Nuclear corepressor KAP-1, RING finger protein 96, RING-type E3 ubiquitin transferase TIF1-beta, Tripartite motif-containing protein 28, KAP-1, KRIP-1

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (AB272068)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (AB272068)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue and serial sections of etoposide treated HeLa cells stained for KAPI (phospho S824) using ab243870 at 1/100 dilution in immunohistochemical analysis. Mock phosphatase treated sections (left) and calf intestinal phosphatase-treated sections (right) are also shown. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243870).

Immunocytochemistry/ Immunofluorescence - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (AB272068)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (AB272068)

Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with etoposide (right) or untreated (left) labeling KAP-1 (phospho S824) (red) using ab243862 at 1/100 dilution in ICC analysis. DyLight® 594-conjugated goat anti-rabbit IgG was used as the secondary antibody. Counterstain : Phalloidin conjugated Alexa Fluor® 488 (green).

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243870).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (AB272068)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (AB272068)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for KAPI (phospho S824) using ab243870 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243870).

Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (AB272068)
  • IP

Unknown

Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (AB272068)

KAP1 (phospho S824) was immunoprecipitated from 1 mg HEK-293T whole cell lysate treated with 100 μM etoposide or untreated, using ab243870 at 20 μl per reaction. Western blot was performed on the immunoprecipitate using ab243870 at 1/1000 dilution.

Lane 1 : ab243870 IP in HEK-293T cell lysate treated with 100 μM etoposide.

Lane 2 : ab243870 Ip in untreated HEK-293T whole cell lysate.

Lane 3 : Control IgG in HEK-293T whole cell lysate treated with 100 μM etoposide.

Detection : Chemiluminescence with an exposure time of 10 seconds

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243870).

All lanes:

Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (<a href='/products/primary-antibodies/kap1-phospho-s824-antibody-bl-246-7b5-ab243870'>ab243870</a>)

Predicted band size: 88 kDa

false

Western blot - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (AB272068)
  • WB

Supplier Data

Western blot - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (AB272068)

All lanes:

Western blot - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068) at 1/1000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate, treated with 100 µM etoposide at 15 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate, mock treated at 15 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG antibody

Predicted band size: 88 kDa

false

Exposure time: 30s

関連する標識済み抗体及び組成の異なる製品 (1)

  • Unconjugated

    Anti-KAP1 (phospho S824) antibody [BL-246-7B5]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

BL-246-7B5

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

IP, WB, IHC-P, ICC/IF

applications

免疫原

Synthetic Peptide within Human TRIM28 pS824. The exact immunogen used to generate this antibody is proprietary information.

Q13263

style
left-column

代替製品

Primary Antibodies

AB3831

Anti-KAP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (AB3831)

  • 5
  • 4
Primary Antibodies

AB22553

Anti-KAP1 antibody [20C1]

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody [20C1] (AB22553)

  • 4
  • 14
Primary Antibodies

AB10483

Anti-KAP1 antibody

Immunoprecipitation - Anti-KAP1 antibody (AB10483)

  • 3
  • 7
Primary Antibodies

AB10484

Anti-KAP1 antibody

Western blot - Anti-KAP1 antibody (AB10484)

  • 5
  • 6
Primary Antibodies

AB243871

Anti-KAP1 antibody [BL-248-2G6]

Recombinant

Immunocytochemistry - Anti-KAP1 antibody [BL-248-2G6] (AB243871)

  • 0
  • 0
Primary Antibodies

AB109545

Anti-KAP1 antibody [EPR5249]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-KAP1 antibody [EPR5249] (AB109545)

  • 2
  • 1
Primary Antibodies

AB109287

Anti-KAP1 antibody [EPR5216]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody [EPR5216] (AB109287)

  • 5
  • 4
Primary Antibodies

AB109289

Anti-KAP1 antibody [EPR5217]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-KAP1 antibody [EPR5217] (AB109289)

  • 0
  • 0
Primary Antibodies

AB133440

Anti-KAP1 (phospho S824) antibody [EPR5248]

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [EPR5248] (AB133440)

  • 5
  • 1
Primary Antibodies

AB215549

Anti-KAP1 (phospho S824) antibody [EPR5248] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [EPR5248] - BSA and Azide free (AB215549)

  • 0
  • 0
Primary Antibodies

AB215550

Anti-KAP1 antibody [EPR5217] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-KAP1 antibody [EPR5217] - BSA and Azide free (AB215550)

  • 0
  • 0
Primary Antibodies

AB84077

Anti-KAP1 (phospho S824) antibody

Immunocytochemistry/ Immunofluorescence - Anti-KAP1 (phospho S824) antibody (AB84077)

  • 0
  • 0
Primary Antibodies

AB70369

Anti-KAP1 (phospho S824) antibody

Immunocytochemistry - Anti-KAP1 (phospho S824) antibody (AB70369)

  • 5
  • 6
Primary Antibodies

AB190178

Anti-KAP1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-KAP1 antibody (AB190178)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB6326

Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

Flow Cytometry (Intracellular) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (AB6326)

  • 5
  • 104
Primary Antibodies

AB81292

Anti-ATM (phospho S1981) antibody [EP1890Y]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (AB81292)

  • 5
  • 18
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB76420

Anti-RPA32/RPA2 antibody [EPR2877Y]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)

  • 5
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p>Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

製品の詳細

ab272068 is the BSA-free version of ab243870.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製に関する特記事項
Recombinant antibody was purified from cell culture supernatant.
バッファー組成
pH: 8.2 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
+4°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

KAP1 also known as TRIM28 or TIF1β is a multifunctional protein involved in transcriptional regulation. It has a molecular weight of approximately 87 kDa. This protein is expressed in a wide variety of human tissues where it plays a role as a co-repressor for KRAB domain-containing zinc finger proteins. KAP1 recruits various epigenetic modifiers to silence transcription and this activity depends on its phosphorylation status. You might explore KAP1 using KAP1 ELISA kits and study its post-translational modifications like phosphorylation in various conditions.
Biological function summary

KAP1 acts as a scaffold protein facilitating the assembly of large protein complexes that include chromatin remodelers and histone deacetylases. It is significant in maintaining chromatin structure and mediating gene silencing. KAP1 phosphorylation serves as a switch between its roles in transcriptional repression and activation. This protein also interacts with heterochromatin protein 1 (HP1) and other TRIM family proteins playing an important role in genomic stability by controlling gene expression and repair mechanisms.

Pathways

KAP1 participates actively in p53 and DNA damage response pathways. In the p53 pathway KAP1 regulates genes involved in the cell cycle and apoptosis acting in conjunction with the p53 protein. Within the DNA damage response KAP1 modulates the repair of double-strand breaks by interacting with proteins like ATM kinase influencing cellular sensitivity to genotoxic stress. These pathways highlight its importance in maintaining cellular homeostasis and response to external stimuli.

KAP1 has known connections with cancer and neurological disorders due to its regulatory effects on gene expression and genome stability. In cancer aberrant KAP1 activity can lead to unregulated cell proliferation and survival often involving changes in its interaction with the p53 protein. In neurological disorders dysregulation of KAP1 is associated with impaired neural development and neurodegeneration. Altogether these associations highlight KAP1 as a potential target for therapeutic interventions in related diseases.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also a corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteasomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg) (PubMed : 23543754). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed : 24623306). Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed : 24623306). In ESCs, in collaboration with SETDB1, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (By similarity). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed : 24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed : 27029610).. (Microbial infection) Plays a critical role in the shutdown of lytic gene expression during the early stage of herpes virus 8 primary infection. This inhibition is mediated through interaction with herpes virus 8 protein LANA1.
See full target information TRIM28 pS824
style
left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 15:1415-1429 PubMed40370549

2025

Effective therapeutic targeting of tumor lineage plasticity in neuroendocrine prostate cancer by BRD4 inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Xiong Zhang,Yatian Yang,Hongye Zou,Yang Yang,Xingling Zheng,Eva Corey,Amina Zoubeidi,Nicolas Mitsiades,Ai-Ming Yu,Yuanpei Li,Hong-Wu Chen
View all publications
style
left-column
style
left-column
style
right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com